| 幼儿园儿童巨细胞病毒分离毒株的限制性酶切图谱分析 |
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| 引用本文： | 王笑峰,罗兵.幼儿园儿童巨细胞病毒分离毒株的限制性酶切图谱分析[J].青岛大学医学院学报,2002,38(1):17-19. |
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| 作者姓名： | 王笑峰 罗兵 |
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| 作者单位： | 青岛大学医学院微生物学教研室,青岛,266021 |
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| 摘 要： | （1）目的：调查幼儿园学龄前儿童间人巨细胞病毒（HCMV）传播的分子流行病学状况。（2）方法：应用限制性核酸内切酶，对112名幼儿园儿童尿本分离到的部分HCMV毒株进行酶切图谱分析。（3）结果：自2所幼儿园同组儿童分离到的大多数HCMV毒株具有相同的限制性酶切图谱。（4）结论：水平传播的幼儿园婴幼儿间HCMV传播的主要方式，婴幼儿彼皮间的密切接触是HCMV传播的重要条件。
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| 关 键 词： | 巨细胞病毒属 限制酶图谱法 学龄前儿童 |
| 文章编号： | 1001-4047(2002)01-0017-03 |
| 修稿时间： | 2001年3月2日 |
RESTRICTION ENDONUCLEASE ANALYSIS OF CYTOMEGALOVIRUS STRAINS ISOLATED FROM CHILDREN ATTENDING KINDERGARTENS |
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| WANG Xiaofeng,LUO Bing \.RESTRICTION ENDONUCLEASE ANALYSIS OF CYTOMEGALOVIRUS STRAINS ISOLATED FROM CHILDREN ATTENDING KINDERGARTENS[J].Acta Academiae Medicinae Qingdao Universitatis,2002,38(1):17-19. |
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| Authors: | WANG Xiaofeng LUO Bing \ |
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| Institution: | WANG Xiaofeng,LUO Bing \ Department of Microbiology,Qingdao University Medical College,Qingdao 266021\, |
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| Abstract: | Objective\ To investigate the molecular epidemiology of human cytomegalovirus(HCMV) among preschool children in kindergartens. \ Methods\ Restriction endonuclease was used to analyze the restriction enzyme cleavage maps for some HCMV strains isolated from urine samples of 112 children in kindergartens.\ Results\ Most of the strains isolated from children of same groups of two kindergartens had same restriction cleavage maps. \ Conclusion\ It is suggested that the horizontal spread of HCMV is a main mode of transmission among children attending nursery schools, close contact among them is an important condition for HCMV spread. |
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| Keywords: | cytomegalovirus restriction endonuclease analysis child preschool |
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