| 流式细胞仪检测人脾LAK细胞 |
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| 引用本文： | 陈宝安,高雪芝,金宝率,邵泽叶,周振英,戴亮,冯平柏,张军妮,朱敏生,许祥裕,朱有华,虞冠华,丁树标,施凤霞.流式细胞仪检测人脾LAK细胞[J].东南大学学报(医学版),1996(1). |
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| 作者姓名： | 陈宝安 高雪芝 金宝率 邵泽叶 周振英 戴亮 冯平柏 张军妮 朱敏生 许祥裕 朱有华 虞冠华 丁树标 施凤霞 |
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| 作者单位： | 南京铁道医学院附属医院血液学实验室，江苏省肿瘤研究所，南京军区军事医学研究所 |
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| 摘 要： | 目的：探求ＬＡＫ细胞对白血病疗效的监测手段。方法：用流式细胞仪测定５例成人脾淋巴因子激活的杀伤细胞（ＬＡＫ细胞）和２１例正常人外周血淋巴细胞的ＤＮＡ指数（ＤＩ）及增殖周期。结果：人脾ＬＡＫ细胞的增殖活力明显高于未激活的脾淋巴细胞和外周血淋巴细胞。培养（１～１４ｄ）期间人脾ＬＡＫ细胞的增殖活力高，并且稳定。在人脾ＬＡＫ细胞中，未找出非整倍体细胞，ＤＩ指数在正常范围。结论：流式细胞仪可用于判断人脾ＬＡＫ细胞对白血病的疗效。
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| 关 键 词： | 淋巴因子激活的杀伤细胞 流式细胞仪 白血病 |
Application of Flow Cytometry to Monitoring the Effect of Allogenic Splenic LAK Cells on Leukemia |
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| Chen Bac''''an,Gao xuezhi,Jin Baocui, Shao Zeye.Application of Flow Cytometry to Monitoring the Effect of Allogenic Splenic LAK Cells on Leukemia[J].Journal of Southeast Univ: Medical Sci Ed,1996(1). |
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| Authors: | Chen Bac'an Gao xuezhi Jin Baocui Shao Zeye |
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| Abstract: | This research was aimed to find out a measure for observing the effect of lymphokine-activated killer (LAK ) cells on leukemia. METHODS: The DNA index (DI) and preliferative cycle of splenic LAK cells from 5 adults and peripheral lymphocytes from 21 normal subjects were estimated with flow cytometry (FCM ). RESULTS: The proliferative activity of splenic LAK cells was significantly higher than that of splenic cells and peripheral lymphocytes. It was highly active and stable during culture for a period of 1-14 d. Non-ploid cells were not found in the splenic LAK cells and the DI remained in normal range. CONCLUSIONS:FCM may be used to monitor the effect of allogenic LAK cells on leukemia. |
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| Keywords: | lymphokine-activated killer cells flow cytometry leukemia |
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