妊娠高血压综合征伴胎儿宫内生长迟缓胎盘11-β羟类固醇脱氢酶的表达

目的探讨胎盘11-β羟类固醇脱氢酶2（11-β HSD2）mRNA表达在妊娠高血压综合征（PIH）伴胎儿宫内生长迟缓（IUGR）中的表达及与新生儿出生体重和胎盘质量间的关系。方法以13例PIH初产妇伴IUGR（PIH伴IUGR组）、22例PIH初产妇伴新生儿为适于胎龄儿（PIH伴AGA组）和36例正常初产妇伴新生儿为适于胎龄儿（对照组）作为研究对象，采用RT—PCR法检测胎盘11-β HSD2mRNA表达水平，采用化学发光法检测脐血清皮质醇水平。结果PIH伴IUGR组胎盘11-β HSD2mRNA表达水平（0．26±0．09）显著低于PIH伴AGA组（0．64±0．19）和对照组（0．66±0．20）；PIH伴IUGR组脐血清皮质醇水平（71．60±20．20）μg／L]显著高于PIH伴AGA组（51．00±13．80）μg／L]和对照组（49．10±14．40）μg／L]；PIH伴IUGR组新生儿出生体重和胎盘质量显著低于PIH伴AGA组和对照组；胎盘11-β HSD2mRNA表达水平与新生儿出生体重和胎盘质量均呈显著正相关。结论PIH伴IUGR胎盘11-β HSD2mRNA低表达，可能导致胎儿体内皮质醇过多而影响胎儿生长发育。

11-β hydroxysteroid dehydrogenase gene expression in placenta of pregnancy induced hypertension complicated by intrauterine growth retardation

Objective To explore the expression of 11-β hydroxysteroid dehydrogenase 2 （ 11-β HSD2 } gene in placenta of pregnancy induced hypertension （PIH）complicated by intrauterine growth retardation （IUGR）and the relationship between different expression of 11-β HSD2 in placenta and newborn＇s birth weight or placental weight. Methods Thirteen cases of term pregnancy mothers with PIH complicated by IUGR were served as PIH complicated by IUGR group, 22 cases of term pregnancy mothers complicated by PIH with appropriate for gestational age （AGA） infant as PIH with AGA group and 36 cases of normal controls as control group. The mRNA expression level of 11-β HSD2 gene in placenta was evaluated by RT-PCR. The level of cord serum cortisol was detected by the method of chemiluminescence. Results The 11- β HSD2 gene mRNA was expressed in placenta. The mRNA expression level of 11- β HSD2 gene in PIH complicated by IUGR group＇s placenta was significantly lower （0.26 ± 0.09） than that in PIH with AGA group （0.64 ± 0.19）and control group （0.66 ± 0.20）. The level of cord serum cortisol in PIH complicated by IUGR group was significantly higher （71.60 ± 20.20） μg/L] than that in PIH with AGA group （51.00 ± 13.80） μg/L land control group （49.10 ± 14.40） μg/L]. The newborn＇s birth weight and placental weight in PIH complicated by IUGR group was significantly lower than those in PIH with AGA group and control group. The mRNA expression level of 11-β HSD2 gene in placenta was positively correlated with the birth weight of their newborns and placental weight. Conclusion The lower mRNA expression level of 11-β HSD2 gene inplacenta may contribute to the higher cortisol level in fetal of PIH complicated by IUGR and has a negative role on the fetal development.