|
|||||
|
|
| 反相高效液相色谱法同时分析龙葵中3种甾体生物碱 | |
| 引用本文： | 袁海建,陈宜刚,蔡宝昌,贾晓斌,陈彦. 反相高效液相色谱法同时分析龙葵中3种甾体生物碱[J]. 中国中药杂志, 2011, 36(12): 1630-1632 |
| 作者姓名： | 袁海建 陈宜刚 蔡宝昌 贾晓斌 陈彦 |
| 作者单位： | 1. 泰州职业技术学院,江苏泰州2253001;江苏省中医药研究院中药新型给药系统重点实验室,江苏南京210028 2. 泰州职业技术学院,江苏泰州,2253001 3. 国家教育部中药炮制规范化及标准化工程研究中心江苏省中药炮制重点实验室,江苏南京,2100292 4. 江苏省中医药研究院中药新型给药系统重点实验室,江苏南京,210028 |
| 基金项目： | 江苏省中医药局项目(LB09079);教育部高等学校青年骨干教师国内访问学者项目 |
| 摘 要： | 目的:利用反相高效液相色谱法快速测定龙葵中3种甾体生物碱的含量.方法:采用Agilent Zorbax SB-C1s(4.6mm×150 mm,5μm)色谱柱,以乙腈-1%磷酸为流动相,流速1.0 mL·min-1,进样量20μL,柱温30℃,205 nm条件下检测,以梯度洗脱方式在30 min内分离了澳洲茄碱、澳洲茄边碱和khasianine 3种生物碱.结果:澳洲茄碱含量测定的线性范围为0.860～10.320μg(r=0.999 7);澳洲茄边碱含量测定的线性范围为0.726～8.710μg(r=0.999 7);khasianine含量测定的线性范围为0.696～8.352μg(r=0.9998).方法的平均回收率分别为100.18%,99.08%,99.88%.结论:方法简便快速、准确度高,可用于龙葵药材的质量评价. |
| 关 键 词： | 反相高效液相色谱法 龙葵 澳洲茄碱 澳洲茄边碱 khasianine |
| 收稿时间： | 2010-09-13 |
Simultaneous determination of three steroidal alkaloids from Solanum Nigrum by RP-HPLC |
|
| YUAN Haijian,CHEN Yigang,CAI Baochang,JIA Xiaobin and CHEN Yan. Simultaneous determination of three steroidal alkaloids from Solanum Nigrum by RP-HPLC[J]. China Journal of Chinese Materia Medica, 2011, 36(12): 1630-1632 | |
| Authors: | YUAN Haijian CHEN Yigang CAI Baochang JIA Xiaobin CHEN Yan |
| Affiliation: | Taizhou polytechnic college, Taizhou 225300, China;Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China;Taizhou polytechnic college, Taizhou 225300, China;Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Jangsu Key Laboratory of Chinese Medicine Processing, Nanjing 210029, China;Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China;Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China |
| Abstract: | |
| Keywords: | reversed-phase high performance liquid chromatography Solanum Nigrum solasonine solamargine khasianine |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |