血小板减少性紫癜患儿T细胞亚群与免疫球蛋白水平检测及分析

目的观察血小板减少性紫癜患儿T细胞亚群与免疫球蛋白的动态变化,以探讨其发病原理及治疗对策。方法采用流式细胞技术和散色比浊法测定血小板减少性紫癜急性期与恢复期及对照组儿童血清T细胞亚群水平和免疫球蛋白浓度。结果血小板减少性紫癜患儿CD3+、CD4+细胞及CD4+/CD8+比值与对照组比较上升(P<0.01、P<0.01、P<0.05),CD8+、CD19+、CD56+均降低(P<0.01、P<0.01、P<0.05),恢复期与对照组之间无显著差异(P>0.05)。患儿血清免疫球蛋白IgM明显高于对照组(P<0.01),IgG明显低于对照组(P<0.01),IgA与对照组无明显变化,恢复期与对照组之间无显著差异(P>0.05)。结论原发性血小板减少性紫癜患儿在体液免疫与细胞免疫方面均有异常改变,且与病情相关。研究提示在治疗时可加用免疫调节剂。

Detection and Analysis of Blood T-cell Subgroup and Immunoglobulin Level of Thrombocytopenic Purpura of Children

Objective To observe the dynamic changes of T-cell subgroup and immunoglobulin level of thrombocytopenic purpura of children so that to discuss the mechanism and its treatment. Methods The immunoglobulin concentration and T-cell subgroup level of thrombocytopenic purpura children in acute stage and convalescent stage were detected with the nephelometer and FCM. Results The contents of CD3^＋, CD4^＋ cell and ratio of CD4^＋/CD8^＋ were higher than those in control group （P 〈0.01, P 〈0.01, P 〈0.05）. The contents of CD8^＋, CD19^＋ and CD56^＋ cell were lower than those in control group （P 〈0.01, P 〈0.01, P 〈0.05）. The contents of CD3^＋, CD4^＋, CD8^＋, CD 19^＋, CD56^＋ cell and ratio of CD4^＋/CD8^＋ of convalescent stage of these children had no significant difference with those in control group. IgM concentration in acute stage of thrombocytopenic purpura children was significantly higher than that in control group （P 〈0.01）. IgG concentration in acute stage of thrombocytopenic purpura children was significantly lower than that in control group （P 〈0.01）. IgA concentration had no significant difference with that in control group. Conclusions There are abnormal changes in body fluid immunity and cell immunity in thrombocytopenic purpura and related with the state of illness. The study demonstrates that the immune regulator can be used in the routine therapy of thrombocytopenic purpura.