银杏叶提取物对糖尿病大鼠体内氧化应激的影响

目的观察银杏叶提取物对糖尿病(DM)大鼠体内氧化应激水平的影响。方法利用Wistar大鼠建立糖尿病模型,将糖尿病大鼠分为糖尿病组(DM)和银杏叶治疗组(GBE),另设正常组动物为对照。DM组和正常对照组大鼠每日通过腹腔注射给予生理盐水0.83ml.kg-1,GBE组大鼠每日通过腹腔注射给予GBE0.83ml.kg-1,连续注射30天后取血测定各组大鼠血清中丙二醛(MDA)含量和超氧阴离子(O2-)水平,然后取出胰腺、肾脏测定MDA含量并采用免疫组化S-P法(链霉素抗生物素蛋白一过氧化物酶)检测肾脏蛋白激酶C(PKC)的表达。结果与正常对照组相比,DM组和GBE组大鼠血清、肾脏和胰腺中MDA的含量及血清中超氧阴离子(O2-)的水平显著升高(P0.05);GBE组大鼠血清、肾脏和胰腺中MDA的含量及超氧阴离子(O2-)的水平显著低于DM组(P0.05)。免疫组织化学染色结果表明,DM组PKC表达显著强于GBE组。结论银杏叶提取物可减轻糖尿病大鼠体内的氧化应激水平,具有抗氧化作用,对糖尿病并发症的发生发展具有一定的预防作用。

Effects of Ginkgo Biloba Extract on the Oxidative Stress in Diabetic Rats

Objective To explore the effects of Ginkgo Biloba extract on the oxidative stress in diabetic rats. Methods Wistar rats were used to establish diabetic model rats, and the diabetic model rats were randomly classified into diabetic group in which the rats were administered saline intraperitoneally at the dose of 0.83 ml· kg- 1, and GBE group in which rats were administered GBE intraperitoneally at the dose of 0.83 ml·kg^-1. Normal rats were used as normal control. After 30 days, the serum in rats from different groups were collected to test the level of superoxide anion and malondialdehyde, the expression of protein kinase C （PKC） in kidney was alsto determined. Results The level of MDA and superoxide anion in rats from diabetic group was significantly higher than those from normal control and GBE group. The expression of PKC in diabetic rats was stronger than that in GBE rats. Conclusion GBE can attenuate the oxidative stress in diabetic rats and play a role in the prevention of diabetic complicaiton development.