Solubilization of HL-A Antigens from Peripheral Blood Leukocytes

Peripheral blood leukocytes, isolated by continuous flow leukapheresis and purification, are an excellent source of HL-A antigen. 3M KCI extraction releases the HL-A antigenic mosaic with good yield and specificity. This technique will allow a selective choice of HL-A representation in source material for studies of biochemical characteristics of the HL-A antigens. The technique would allow preparation of antigen from a proposed allograft donor and the pretreatment of the recipient in attempts to induce specific immunoIogic unresponsiveness.