HPLC法测定双黄连口服液中黄芩苷与连翘苷的含量研究

目的采用高效液相色谱法测定双黄连口服液中黄芩苷与连翘苷的含量。方法黄芩苷:色谱柱为Kromasil ODS柱(4.6mm×150mm,5μm);柱温:40℃;检测波长280nm;流动相:甲醇-水-磷酸(55∶45∶0.2);流速:1.0mL·min-1。连翘苷:色谱柱为Kromasil ODS柱(4.6mm×150mm,5μm);检测波长277nm;流动相:乙腈-水(25∶75);流速:0.8mL·min-1。结果黄芩苷在24.85~198.8mg·L-1间与峰面积有良好的线性关系,连翘苷进样量在0.496~3.472μg范围内与峰面积呈良好线性关系;加样回收率黄芩苷为101.1%,RSD=1.83%(n=5),连翘苷为98.71%,RSD=1.61%(n=5)。结论该方法简单迅速,结果准确,精密度好,对黄芩苷和连翘苷的含量测定能更好的控制双黄连口服液的质量。

Determination of baicilin and forsythin in Shuanghuanglian Oral Liquid by HPLC

OBJECTIVE HPLC method was used to determine the content of baicalin and forsythin in Shuanghuanglian Oral Liquid.METHODS The chromatograph to determine baicalin consisted of Kromasil ODS column(4.6mm×150mm,5μm) with temperature 40 ℃ at the detective wavelength of 280nm, the mobile phase was methanol-water-phosphoric acid(55∶45∶0.2) with the flow rate of 1.0 mL·min-1.The chromatograph of forsythin Kromasil ODS column(4.6mm×150mm,5μm)with mobile phase of acetonitrile-water(25∶75),the detective wavelength was 277nm,the flow rate was 0.8 mL·min-1.RESULTS Baicalin showed a good linear relationship at the range of 24.85～198.8 mg·L-1, forsythin liked that at the range of 0.496 ～3.472 μg, the average recovery of baicalin was 101.1%, and that of forsythin was 1.83%.CONCLUSION The method was simple,quick,accurate and had good precision,it can be used to control the quality of Shuanghuanglian Oral Liquid.