HPLC法测定芩杏清热止咳颗粒中8种成分

目的建立HPLC法测定芩杏清热止咳颗粒(黄芩、栀子、桑叶等)中8种成分的含有量。方法黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的分析采用Diamonsil Plus C₁₈色谱柱(4.6 mm×250 mm,5μm);流动相0.2%磷酸-甲醇,梯度洗脱;体积流量1.0 mL/min;柱温25℃;检测波长274 nm。栀子苷、甘草苷、异甘草苷、甘草酸的分析采用Diamonsil Plus C₁₈色谱柱(4.6 mm×250 mm,5μm);流动相0.05%磷酸-乙腈,梯度洗脱;体积流量1.0 mL/min;柱温25℃;检测波长237、360 nm。结果 8种成分在各自范围内线性关系良好(r>0.999 5),平均加样回收率97.35%～98.73%,RSD 0.61%～1.9%。结论该方法准确可靠,重复性好,可用于芩杏清热止咳颗粒的质量控制。

Determination of eight constituents in Qinxing Qingre Zhike Granules by HPLC

AIM To establish an HPLC method for the content determination of eight constituents in Qinxing Qingre Zhike Granules(Scutellariae Radix,Gardeniae Fructus,Mori Folium,etc.).METHODS The analyses of baicalin,wogonoside,baicalein and wogonin were performed on a 25℃thermostatic Diamonsil Plus C18column(4.6 mm×250 mm,5μm),with the mobile phase comprising of 0.2%phosphoric acid-methanol flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 274 nm.The analyses of geniposide,liquritin,isoliquiritin and glycyrrhizic acid were performed on a 25℃thermostatic Diamonsil Plus C18column(4.6 mm×250 mm,5μm),with the mobile phase comprising of 0.05%phosphoric acid-acetonitrile flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 237,360 nm.RESULTS Eight constituents showed good linear relationships within their own ranges(r>0.999 5),whose average recoveries were 97.35%-98.73%with the RSDs of 0.61%-1.9%.CONCLUSION This accurate,reliable and reproducible method can be used for the quality control of Qinxing Qingre Zhike Granules.