| 杨桃根多糖提取工艺优化及其体外活性 |
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| 引用本文： | 廖彭莹,李典鹏,扈芷怡,陈慧萍,杨小妹,吴勇燕. 杨桃根多糖提取工艺优化及其体外活性[J]. 中成药, 2019, 0(9): 2030-2034 |
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| 作者姓名： | 廖彭莹 李典鹏 扈芷怡 陈慧萍 杨小妹 吴勇燕 |
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| 作者单位： | 1.广西中医药大学;2.广西壮族自治区中国科学院广西植物研究所广西植物功能物质研究与利用重点实验室 |
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| 基金项目： | 广西自然科学基金(2015GXNSFBA139116);中国科学院植物资源保护与可持续利用重点实验室开放课题(PCU201503);广西植物功能物质研究与利用重点实验室2016年开放基金(FPRU2016-13);广西中医药大学药学院中药学学科青年教师提升计划(2017);广西中医药大学2017年自治区级大学生创新创业训练计划(201710600070);广西中医药大学2018年广西一流学科建设项目重点课题(2018XK045) |
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| 摘 要： | 目的优化杨桃Averrhoa carambola L.根多糖提取工艺,并评价其体外活性。方法在单因素试验基础上,以提取温度、提取时间、料液比为影响因素,多糖提取率为评价指标,正交试验优化提取工艺。然后,酶标板法考察多糖抗氧化活性及其对α-葡萄糖苷酶的抑制作用。结果最佳条件为提取温度70℃,提取时间30 min,料液比1∶30,多糖提取率1.93%。多糖对ABTS^·+、DPPH·的EC50值分别为0.10、0.33 mg/mL,对α-葡萄糖苷酶的抑制率高于阿卡波糖。结论该方法稳定可靠,可用于提取具有较强抗氧化活性、抑制α-葡萄糖苷酶作用的杨桃根多糖。
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| 关 键 词： | 杨桃 根 多糖 提取 抗氧化活性 Α-葡萄糖苷酶 正交试验 酶标板法 |
Extraction process optimization and in vitro activity evaluation for polysaccharides from Averrhoa carambola roots |
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| LIAO Peng-ying,LI Dian-peng,HU Zhi-yi,CHEN Hui-ping,YANG Xiao-mei,WU Yong-yan. Extraction process optimization and in vitro activity evaluation for polysaccharides from Averrhoa carambola roots[J]. Chinese Traditional Patent Medicine, 2019, 0(9): 2030-2034 |
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| Authors: | LIAO Peng-ying LI Dian-peng HU Zhi-yi CHEN Hui-ping YANG Xiao-mei WU Yong-yan |
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| Affiliation: | (Guangxi University of Chinese Medicine,Nanning 530001,China;Guangxi Key Laboratory for Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin 541006,China) |
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| Abstract: | AIM To optimize the extraction process for polysaccharides from Averrhoa carambola L. roots and to evaluate their in vitro activities.METHODS With extraction temperature, extraction time and solid-liquid ratio as influencing factors, the extraction rate of polysaccharides as an evaluation index, the extraction process was optimized by orthogonal test on the basis of single factor test. Subsequently, enzyme label plate assay was applied to investigating polysaccharides’ antioxidant activity and inhibitory effect on α-glucosidase.RESULTS The optimal conditions were determined to be 70 ℃ for extraction temperature, 30 min for extraction time, and 1∶30 for solid-liquid ratio, the extraction rate of polysaccharides was 1.93%. The EC50 values of polysaccharides on ABTS^·+, DPPH·were 0.10, 0.33 mg/mL, respectively, whose inhibitory rate on α-glucosidase was higher than that of acarbose.CONCLUSION This stable and reliable method can be used for extracting polysaccharides, with strong antioxidant activity and inhibitory effect on α-glucosidase from A. carambola roots. |
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| Keywords: | Averrhoa carambola L. roots polysaccharides extraction antioxidant activity α-glucosidase orthogonal test enzyme label plate assay |
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