| 甜茶质量标准的研究 |
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| 引用本文： | 刘刚,周志英,刘育辰,张永萍,杨立勇,向兴泉,林泽武.甜茶质量标准的研究[J].中成药,2019(10):2324-2327. |
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| 作者姓名： | 刘刚 周志英 刘育辰 张永萍 杨立勇 向兴泉 林泽武 |
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| 作者单位： | 1.贵州中医药大学药学院;2.国家苗药工程技术研究中心;3.贵州特色制药有限责任公司 |
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| 基金项目： | 贵州省中药材、民族药材质量标准项目(DBYC52164);贵州省中药、民族药饮片标准项目(DBYP52289) |
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| 摘 要： | 目的建立甜茶Rubus suavissimus S. Lee的质量标准。方法 TLC法定性鉴别芦丁、金丝桃苷,HPLC法测定金丝桃苷含有量,药典法测定水分、醇溶性浸出物含有量。结果 TLC斑点清晰,重复性好;金丝桃苷在0.01~0.2 mg/mL范围内呈良好的线性关系(R^2=0.999 9),平均加样回收率为100.05%,RSD为0.43%。10批样品中,水分、醇溶性浸出物含有量范围分别为7.35%~9.19%、26.04%~45.11%。结论该方法稳定可靠,可用于甜茶的质量控制。
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| 关 键 词： | 甜茶 芦丁 金丝桃苷 TLC HPLC |
Quality standard for Rubus suavissimus |
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| LIU Gang,ZHOU Zhi-ying,LIU Yu-chen,ZHANG Yong-ping,YANG Li-yong,XIANG Xing-quan,LIN Ze-wu.Quality standard for Rubus suavissimus[J].Chinese Traditional Patent Medicine,2019(10):2324-2327. |
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| Authors: | LIU Gang ZHOU Zhi-ying LIU Yu-chen ZHANG Yong-ping YANG Li-yong XIANG Xing-quan LIN Ze-wu |
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| Institution: | (College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China;National Engineering and Technology Research Center for Miao Medicine, Guiyang 550025, China;Guizhou Characteristic Pharmaceutical Co., Ltd., Guiding 551300, China) |
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| Abstract: | AIM To establish the quality standard for Rubus suavissimus S. Lee. METHODS TLC was used for the qualitative identification of rutin and hyperoside. HPLC was adopted in the content determination of hyperoside and water, and pharmacopeia method was applied to determining water and alcohol-soluble extract contents. RESULTS The clear TLC plots were reproducible. Hyperoside showed a good linear relationship within the range of 0.01-0.2 mg/mL(R^2=0.999 9), whose average recovery was 100.05% with the RSD of 0.43%. In ten batches of samples, the content ranges of water and alcohol-soluble extract were 7.35%-9.19% and 26.04%-45.11%, respectively. CONCLUSION This stable and reliable method can be used for the quality control of R. suavissimus. |
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| Keywords: | Rubus suavissimus S Lee rutin hyperoside TLC HPLC |
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