RASSF10基因启动子区甲基化状态在宫颈癌中的临床应用

目的探讨RASSF10基因启动子区甲基化状态在宫颈癌中的临床意义。方法收集本院2005年6月～2013年12月经术后病理证实的70例宫颈癌患者,取其经手术切除癌组织70例和配对癌旁组织标本70例,采用甲基化特异性PCR(MSP)分析法检测RASSF10基因启动子区DNA甲基化状态,分析RASSF10基因启动子区甲基化状态与临床病理特征(年龄、肿瘤大小、颈深部间质侵犯、盆腔淋巴结转移、FIGO分期及肿瘤分级程度)的关系及宫颈癌预后的相关性因素分析。结果 MSP检测发现宫颈癌组织中RASSF10基因启动子区甲基化为38.6%,未甲基化为43例(61.4%),而正常宫颈组织未见甲基化;宫颈癌中RASSF10基因启动子甲基化状态与年龄、肿瘤大小、颈深部间质侵犯及盆腔淋巴结转移无关(P0.05),而与FIGO分期及肿瘤分化程度有关。70例宫颈癌患者的中位生存期与年龄、肿瘤大小及颈深部间质侵犯无关,但与FIGO分期、肿瘤分化程度、盆腔淋巴结转移及RASSF10基因启动子区甲基化有关,其中RASSF10基因启动子区甲基化者5年生存率为66.7%,未甲基化者的5年生存率为88.4%,差异具有统计学意义(P0.05)。多因素分析结果显示FIGO分期、肿瘤分化程度、盆腔淋巴结转移和RASSF10基因启动子区甲基化状态为本组宫颈癌患者预后独立因素。结论 RASSF10基因启动子区甲基化在宫颈癌中高表达,且与FIGO分期、肿瘤分化程度及预后有关,可作为宫颈癌预后的参考指标。

Clinical application of methylation status of RASSF10 gene promoter region in cervical cancer

Objective To investigate the clinical significance of methylation status of RASSF10 gene promoter region in cervical cancer.Methods Seventy patients with cervical cancer who were confirmed by postoperative pathology from June 2005 to December 2013 were enrolled.70 cases of surgically resected cancer tissues and 70 matched paracancerous tissues were selected for methylation-specific PCR.Methylation specific PCR(MSP)assay was used for DNA methylation status in the promoter region of RASSF10 gene.The relationship between the methylation status of RASSF10 gene promoter region and clinicopathological features(age,tumor size,deep cervical interstitial invasion,pelvic lymph node metastasis,FIGO stage and tumor grade)was analyzed.And the correlation factors of cervical cancer prognosis were analyzed.Results MSP assay showed that the methylation rate of promoter region of RASSF10 gene was 38.6%in cervical cancer tissues,and 43 cases(61.4%)were unmethylated,while no methylation was observed in normal cervical tissues.Methylation status of RASSF10 gene promoter in cervical cancer was not associated with age,tumor size,deep cervical interstitial invasion and pelvic lymph node metastasis(P>0.05),but was related to FIGO stage and tumor grade.The median survival of 70 patients with cervical cancer was not associated with age,tumor size,and deep interstitial invasion of the neck,but was associated with FIGO stage,tumor grade,pelvic lymph node metastasis,and methylation of the RASSF10 gene promoter region,in which the 5-year survival rate of RASSF10 gene promoter region was 66.7%,and the 5-year survival rate of unmethylated patients was 88.4%.The difference was statistically significant(P<0.05).Multivariate analysis showed that FIGO stage,tumor differentiation,pelvic lymph node metastasis and methylation status of RASSF10 gene promoter region were independent prognostic factors for cervical cancer patients.ConclusionThe promoter methylation of RASSF10 gene is highly expressed in cervical cancer,and it is related to FIGO stage,tumor differentiation and prognosis.It can be used as a reference index for prognosis of cervical cancer.