冬瓜皮炭对慢性肾衰竭大鼠肾功能的保护作用

目的探讨冬瓜皮炭对慢性肾衰竭大鼠肾功能的保护作用。方法 72只大鼠随机分为正常组、模型组、阳性对照组(爱西特,450 mg/kg)及冬瓜皮炭低、中、高剂量组(300、450、600 mg/kg)。腺嘌呤灌胃给药30 d后,ELISA法检测血清BUN、SCr、TGF-β1、PAI-1水平,HE染色、Masson染色观察肾组织病理形态变化,RT-PCR检测NF-κB p65、I-κB mRNA表达,Western blot检测NF-κB p65蛋白表达。结果与模型组比较,冬瓜皮炭高剂量组BUN、SCr、TGF-β1、PAI-1水平,NF-κB p65、I-κB mRNA表达,NF-κB p65蛋白表达显著降低(P<0.05,P<0.01),肾小管轻度扩张、肾小管上皮细胞轻度空泡样变性、肾间质纤维化、炎细胞浸润明显缓解。结论冬瓜皮炭(600 mg/kg)能降低慢性肾功能衰竭大鼠肾脏有害指标,改善肾功能,其机制可能与干预NF-κB信号通路有关。

Protective effects of Benincasae Exocarpium charcoal on renal functions in rats with chronic kidney failure

AIM To explore the protective effects of Benincasae Exocarpium charcoal on renal functions in rats with chronic kidney failure.METHODS Seventy-two rats were randomly divided into normal group, model group, positive control group(aceset, 450 mg/kg) and low-dose, medium-dose, high-dose Benincasae Exocarpium charcoal groups(300, 450, 600 mg/kg). After 30-day intragastric administration of adenine, serum BUN, SCr, TGF-β1 and PAI-1 levels were detected by ELISA, the pathological morphology of renal tissues was observed by HE staining and Masson staining. NF-κB p65 and I-κB mRNA expressions were detected by RT-PCR, NF-κB p65 protein expression was detected by Western blot. RESULTS Compared with the model group, the high-dose Benincasae Exocarpium charcoal group demonstrated significantly decreased BUN, SCr, TGF-β1 and PAI-1 levels, NF-κB p65 and I-κB mRNA expressions, and NF-κB p65 protein expression(P<0.05, P<0.01), with obviously alleviated mild tubular dilatation, mild vacuolar degeneration of renal tubular epithelial cells, renal interstitial fibrosis and decreased inflammatory cell infiltration.CONCLUSION Benincasae Exocarpium charcoal(600 mg/kg) can reduce renal harmful indices and improve renal functions in rats with chronic kidney failure, whose mechanisms may contribute to the intervention of NF-κB signaling pathway.