八味龙钻颗粒HPLC指纹图谱建立及7种成分测定

目的建立八味龙钻颗粒(飞龙掌血、大钻、九龙藤等)HPLC指纹图谱,并测定没食子酸、原儿茶酸、青藤碱、红景天苷、儿茶素、橙皮苷、补骨脂素的含有量。方法该药物70%甲醇提取液的分析采用Phenomenex Gemini C18色谱柱(250 mm×4.6 mm, 5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1 mL/min;检测波长245、270 nm;柱温30℃。结果 10批样品指纹图谱中有12个共有峰,相似度大于0.989。7种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率98.5%~103.6%,RSD 0.92%~1.7%。结论该方法简便快速,准确可靠,可用于八味龙钻颗粒的质量控制。

Establishment of HPLC fingerprints of Bawei Longzuan Granules and determination of seven constituents

AIM To establish the HPLC fingerprints of Bawei Longzuan Granules [Toddalia asiatica Lam., Kadsura coccinea(Lem.) A.C. Smith, Bauhinia championii(Benth.) Benth., etc.] and to determine the contents of gallic acid, protocatechuic acid, sinomenine, salidroside,(+)-catechin, hesperidin and psoralen.METHODS The analysis of 70% methanol extract of this drug was performed on a 30 ℃ thermostatic Phenomenex Gemini C18 column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelengths were set at 245, 270 nm.RESULTS There were twelve common peaks in the fingerprints of ten batches of samples with the similarities of more than 0.989. Seven constituents showed good linear relationships within their own ranges(r>0.999 0), whose average recoveries were 98.5%-103.6% with the RSDs of 0.92%-1.7%.CONCLUSION This simple, rapid, accurate and reliable method can be used for the quality control of Bawei Longzuan Granules.