高效液相色谱-荧光检测法测定性早熟女童血浆中双酚A和4-壬基酚的含量

目的:建立高效液相色谱-荧光检测法测定性早熟女童血浆中双酚A和4-壬基酚含量的方法。方法:采用Shim-pack VPODS C18色谱柱(150 mm×4. 6 mm,5μm),流动相为乙腈∶水=70∶30,流速1 m L/min,柱温30℃,检测激发波长275 nm,发射波长315 nm,外标法定量检测血浆中双酚A和4-壬基酚含量。结果:双酚A和4-壬基酚分别在1. 45~5 937. 50 ng/m L (r^2=0. 999 9,n=7)、1. 53~6 250. 00 ng/m L(r^2=0. 998 8,n=7)范围内线性关系良好;日内、日间RSD均<7. 83%,方法回收率均> 94. 6%。结论:本法简便、快速、灵敏、准确,适用于同时测定性早熟女童血浆中双酚A和4-壬基酚的含量和临床血样常规检测。

Determination of Bisphenol A and 4-Nonylphenol in Plasma of Girls with Precocious Puberty by High Performance Liquid Chromatography-Fluorescence Detection

Objective: To establish the high performance liquid chromatography-fluorescence detection( HPLC-FLD) method for determination of bisphenol A( BPA) and 4-nonylphenol( 4-NP) in plasma of of girls with precocious puberty. Methods: Shim-pack VP-ODS C18 chromatographic column( 150 mm×4. 6 mm,5 μm) was adopted,the mobile phase was acetonitrile ∶ water = 70 ∶ 30,with flow rate of 1 m L/min,column temperature of 30 ℃,detection excitation wavelength of 275 nm,and emission wavelength of 315 nm.Content of BPA and 4-NP in plasma was detected by external standard method. Results: The linear relationship between BPA and 4-NP was good in the range from 1. 45 to 5,937. 50 ng/m L( r^2= 0. 999,9,n = 7) and from 1. 53 to 6,250. 00 ng/m L( r^2= 0. 998,8,n = 7).The RSD of intra-day and inter-day precision was < 7. 83%,and the recovery rate was > 94. 6%. Conclusion: This method is simple,rapid,sensitive and accurate,and is suitable for simultaneous determination of BPA and 4-NP in plasma and routine detection of clinical blood samples in girls with precocious puberty.