| UHPLC法同时测定参麦注射液中8种人参皂苷 |
| |
| 引用本文： | 杨林,徐晓珍,陈劲柏,吴友苹,陈娇婷,辛艳飞.UHPLC法同时测定参麦注射液中8种人参皂苷[J].中成药,2019(1):27-30. |
| |
| 作者姓名： | 杨林 徐晓珍 陈劲柏 吴友苹 陈娇婷 辛艳飞 |
| |
| 作者单位： | 1.浙江省医学科学院安全性评价研究中心;2.浙江工业大学长三角绿色制药协同创新中心;3.绍兴市中医院;4.浙江大学医学院附属儿童医院 |
| |
| 基金项目： | 浙江省自然科学基金资助项目(LY17H310009) |
| |
| 摘 要： | 目的建立UHPLC法同时测定参麦注射液(红参、麦冬)中人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2、Rd、Rg3的含有量。方法该药物的分析采用Agilent Eclipse Plus C18色谱柱(2. 1 mm×50 mm,1. 8μm);流动相乙腈-水,梯度洗脱;体积流量0. 3 mL/min;柱温30℃;检测波长203 nm。结果 8种人参皂苷在各自范围内线性关系良好(r>0. 997 0),平均加样回收率97. 43%～104. 24%,RSD 0. 37%～5. 34%。结论该方法稳定可靠,重复性好,可用于参麦注射液质量控制。
|
| 关 键 词： | 参麦注射液 人参皂苷 UHPLC |
Simultaneous determination of eight ginsenosides in Shenmai Injection by UHPLC |
| |
| YANG Lin,XU Xiao-zhen,CHEN Jin-bai,WU You-ping,CHEN Jiao-ting,XIN Yan-fei.Simultaneous determination of eight ginsenosides in Shenmai Injection by UHPLC[J].Chinese Traditional Patent Medicine,2019(1):27-30. |
| |
| Authors: | YANG Lin XU Xiao-zhen CHEN Jin-bai WU You-ping CHEN Jiao-ting XIN Yan-fei |
| |
| Institution: | (Center for Safety Evaluation,Zhejiang Provincial Academy of Medical Sciences,Hangzhou 310013,China;Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals,Zhejiang University of Technology,Hangzhou 310013,China;Shaoxing Hospital of Traditional Chinese Medicine,Shaoxing 312499,China;Children’s Hospital Affiliated to Zhejiang University School of Medicine,Hangzhou 310006,China) |
| |
| Abstract: | AIM To establish a UHPLC method for the simultaneous content determination of ginsenosides Rg1,Re,Rf,Rb1,Rc,Rb2,Rd,Rg3 in Shenmai Injection( Ginseng Radix et Rhizoma Rubra,Ophiopogonis Radix). METHODS The analysis of this drug was performed on a 30 ℃ thermostatic Agilent Eclipse Plus C18column( 2. 1 mm × 50 mm, 1. 8 μm), with the mobile phase comprising of acetonitrile-water flowing at0. 3 mL/min in a gradient elution manner,and the detection wavelength was set at 203 nm. RESULTS Eight ginsenosides showed good linear relationships within their own ranges( r>0. 997 0),whose average recoveries were97. 43%-104. 24% with the RSDs of 0. 37%-5. 34%. CONCLUSION This stable,reliable and reproducible method can be used for the quality control of Shenmai Injection. |
| |
| Keywords: | Shenmai Injection ginsenosides UHPLC |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
