LncRNA SChLAP1调节前列腺癌的凋亡作用及其机制研究 |
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基金项目: | 浙江省医药卫生科技计划项目(2019KY456);浙江省温州市公益性科技计划项目(Y20170231) |
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摘 要: | 目的研究LncRNA SCh LAP1调节前列腺癌细胞凋亡的可能分子机制。方法采用慢病毒转染前列腺癌PC-3细胞,并用qPCR检测转染率;利用凋亡检测试剂盒检测转染PC-3细胞的凋亡情况,通过生物信息学确定其下游的可能靶点。结果在不同靶点病毒作用下PC-3细胞可以下调SCh LAP1的表达,其中3#位点的下调效率最大达40%。PC-3细胞下调后,与阴性对照组相比细胞凋亡比例明显上升,差异有统计学意义。结论 SCh LAP1可以调节前列腺癌细胞的凋亡,而miR-101-5p是其下游的靶点之一。
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关 键 词: | 前列腺癌;LncRNA;SChLAP1;凋亡 |
Study on the role of LncRNA SChLAP1 in regulating apoptosis of prostate cancer and its mechanism |
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Abstract: | Objective]?To investigate the possible molecular mechanism of LncRNA SChLAP1 in the regulation of apoptosis in prostate cancer cells.Methods?Prostate cancer PC-3 cells were transfected with lentivirus,and the transfection rate was detected by qPCR.The apoptosis of PC-3 cells was detected by apoptosis detection kit,and the possible downstream targets were determined by bioinformatics.Results?PC-3 cells could down-regulate the expression of SChLAP1 under the action of different target viruses,and the down-regulation efficiency of 3#locus was up to 40%.After PC-3 cells were down-regulated,the proportion of apoptosis was significantly higher than that of the negative control group,and there was a statistical difference.ConclusionSChLAP1 can regulate the apoptosis of prostate cancer cells,and miR-101-5p is one of its downstream targets. |
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Keywords: | Prostate cancer LncRNA SChLAP1 Apoptosis |
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