七十味珍珠丸对脑缺血再灌注损伤大鼠血脑屏障的保护作用

目的考察七十味珍珠丸对脑缺血再灌注损伤大鼠血脑屏障的保护作用。方法线栓法建立大鼠脑缺血模型,2 h后再灌注。72只大鼠随机分为假手术组、模型对照组、尼莫地平片组(30.00 mg/kg)、七十味珍珠丸组(66.68 mg/kg),每组再分为3个亚组(脑梗死率、血脑屏障通透性、脑组织形态学),于脑缺血再灌注后2、24 h观察神经行为学变化,24 h检测脑梗死率、血清SOD活性和MDA水平、血脑屏障通透性、缺血侧脑组织病理变化和尼氏小体数。结果与模型对照组比较,七十味珍珠丸组显著降低大鼠脑梗死率(P<0.01)和缺血侧脑组织伊文思蓝(EB)含有量(P<0.05),显著改善神经行为学异常(P<0.01),显著增加尼氏小体数(P<0.05),脑组织病理变化、SOD活性、MDA水平有改善趋势,但无显著性差异(P>0.05)。结论七十味珍珠丸对脑缺血再灌注损伤大鼠脑组织有明显保护作用,其机制可能与抑制血脑屏障通透性增加有关。

Protective effects of Qishiwei Zhenzhu Pills on blood-brain barrier in cerebral ischemia-reperfusion injury rats

AIM To investigate the protective effects of Qishiwei Zhenzhu Pills on blood-brain barrier in cerebral ischemia-reperfusion injury rats.METHODS Suture-occluded method was applied to establishing the rat model for cerebral ischemia,followed by reperfusion after 2 h.Seventy-two rats were randomly divided into sham-operation group,model control group,Nimodipine Tablets group(30.00 mg/kg)and Qishiwei Zhenzhu Pills group(66.68 mg/kg),each group was further divided into three subgroups(cerebral infarction rate,blood-brain barrier permeability and brain histomorphology).At 2,24 h after cerebral ischemia-reperfusion,neurobehavioral changes were observed;at 24 h,cerebral infarction rate,SOD activity and MDA level in serum,blood-brain barrier permeability,pathological changes and Nissl body count in ischemic brain tissue were detected.RESULTS Compared with the model control group,the Qishiwei Zhenzhu Pills group demonstrated significantly decreased cerebral infarction rate(P<0.01)and Evans blue(EB)content in ischemic brain tissue(P<0.05),markedly improved neurobehavioral abnormalities(P<0.01),obviously increased Nissl body count(P<0.05),and improvement trends in brain tissue pathological changes,SOD activity,MDA level without significant differences(P>0.05).CONCLUSION Qishiwei Zhenzhu Pills exhibit obvious protective effects on brain tissue in cerebral ischemia-reperfusion injury rats,whose mechanism may contribute to the inhibition of increase of blood-brain barrier permeability.