槲皮素对SW620细胞迁移和侵袭的影响

目的探索槲皮素对结直肠癌细胞SW620迁移和侵袭的影响。方法不同浓度(0、25、50、100μmol/L)槲皮素处理SW620细胞后,MTT法检测细胞存活率,Transwell法检测细胞迁移和侵袭,RT-PCR、Western blot分别检测STAT3 mRNA、蛋白表达。结果槲皮素呈剂量和时间依赖性地降低SW620细胞存活率、STAT3 mRNA表达。最佳浓度、处理时间分别为50μmol/L、48 h,细胞迁移和侵袭显著降低(P<0.05)。敲低STAT3,SW620细胞存活率、迁移和侵袭数目明显减少(P<0.05)。STAT3过表达显著逆转槲皮素对细胞存活率、迁移和侵袭的抑制作用(P<0.05)。结论槲皮素可通过调控STAT3表达来抑制SW620细胞迁移和侵袭。

Effects of quercetin on the migration and invasion of SW620 cells

AIM To explore the effects of quercetin on migration and invasion of colorectal cancer SW620 cells.METHODS SW620 cells were treated with different concentrations(0, 25, 50, 100 μmol/L) of quercetin, after which cell viability was detected by MTT assay, cell migration and invasion were detected by Transwell assay, STAT3 mRNA and protein expressions were detected by RT-PCR and Western blot, respectively.RESULTS Quercetin decreased SW620 cell viability and STAT3 mRNA expression in dose-dependent and time-dependent mannerso the optimal concentration and treatment time were determined to be 50 μmol/L and 48 h, repsectively, along with significantly reduced cell migration and invasion(P<0.05);and the cell viability,migration and invasion of SW620 cells were significantly redued when STAT3 was knocked down(P<0.05). The overexpression of STAT3 obviously reversed the inhibitory effects of quercetin on cell viability, migration and invasion(P<0.05). CONCLUSION Quercetin can inhibit the migration and invasion of SW620 cells by regulating STAT3 expression.