北葶苈子化学成分的研究

目的研究北葶苈子的化学成分。方法采用Dianion HP-20、Toyopearl HW-40柱、ODS、Sephadex LH-20、半制备液相等方法对北葶苈子水提物进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到19个化合物,分别鉴定为3,5-二羟基-4-甲氧基苯甲酸(1)、原儿茶酸甲酯(2)、对羟基苯甲酸(3)、1,2-benzenedicarboxylic acid-1,2-bis [2-(2-hydroxyethoxy) ethyl] ester (4)、苄基-β-D-葡萄糖苷(5)、2-苯乙基-1-O-β-D-吡喃葡萄糖苷(6)、对羟基苯乙腈(7)、邻羟基苯乙腈(8)、N-乙酰基色氨酸(9)、尿嘧啶核苷(10)、methyl dioxindole 3-acetate (11)、dioxindole-3-acetic acid (12)、环(L-脯氨酸-L-苯丙氨酸)(13)、4-amino-4-carboxychroman-2-one (14)、槲皮素-3-O-β-D-吡喃半乳糖苷(15)、异鼠李素-3-O-β-D-吡喃半乳糖苷(16)、山柰酚-3-O-β-D-半乳糖苷(17)、三叶苷(18)、根皮素-2',4'-二-O-β-D-吡喃葡萄糖苷(19)。结论化合物1～9、11～19为首次从该植物中分离得到。

Chemical constituents from the seeds of Lepidium apetalum

AIM To study the chemical constituents from the seeds of Lepidium apetalum Willd.. METHODS The aqueous extract from the seeds of L. apetalum was isolated and purified by Dianion HP-20,Toyopearl HW-40 column,ODS,Sephadex LH-20 and semi-preparative column,then the structures of obtained compounds were identified by physicochemical properties and spectral data. RESULTS Nineteen compounds were isolated and identified as 3,5-dihydroxy-4-methoxy-benzoic acid( 1),protocatechuic acid methyl ester( 2),1,2-benzenedicarboxylic acid-1,2-bis [2-( 2-hydroxyethoxy) ethyl] ester( 3),benzyloxy-1-O-β-D-glucopyranoside( 4),2-phenylethy-1-O-β-D-glucopyranoside( 5),4-hydroxybenzyl cyanide( 6),2-hydroxy-benzeneacetonitrile( 7),Nacetyltryptophan( 8),uridine( 9),2-hydroxybenzamide( 10),methyl dioxindole 3-acetate( 11),dioxindole-3-acetic acid( 12),cyclo( L-Pro-L-Phe)( 13),4-amino-4-carboxychroman-2-one( 14),quercetin-3-O-β-D-galactopyranoside( 15), isorhamnetin-3-O-β-D-galactopyranoside( 16), kaempferol-3-O-β-D-galactopyranoside( 17),trilobatin( 18),phloretin-2’,4’-di-O-β-D-glucopyranoside( 19). CONCLUSION Compounds 1-9,11-19 are isolated from this plant for the first time.