| 超临界CO_2萃取紫苏叶挥发油及其成分分析 |
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| 引用本文： | 金建忠.超临界CO_2萃取紫苏叶挥发油及其成分分析[J].药物分析杂志,2011(5). |
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| 作者姓名： | 金建忠 |
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| 作者单位： | 浙江树人大学生物与环境工程学院; |
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| 摘 要： | 目的:采用超临界流体萃取技术提取紫苏叶挥发油,并用GC-MS对挥发油进行化学成分分析。方法:通过单因素实验和正交实验法确定超临界CO2流体萃取紫苏叶挥发油的最佳条件,考察萃取压力、温度、动态萃取时间及CO2流量对挥发油得率的影响;利用GC-MS分析最佳萃取条件下所得挥发油的化学成分,面积归一化法测定其百分含量。结果:紫苏叶超临界CO2萃取最佳条件为:萃取压力20 MPa,温度35℃,萃取时间150 min,CO2流量10 kg.h-1;挥发油的得率3.2%,从中鉴定出了16个化合物,其含量占出峰物质总量的97.68%。结论:紫苏叶挥发油中富含醚、萜类化合物、酯、酮和醇等组分。
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| 关 键 词： | 紫苏叶 挥发油 超临界流体萃取 GC-MS 正交实验 |
Supercritical CO_2 extraction and analysis of volatile oil of Perilla frutescens leaves |
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| JIN Jian-zhong.Supercritical CO_2 extraction and analysis of volatile oil of Perilla frutescens leaves[J].Chinese Journal of Pharmaceutical Analysis,2011(5). |
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| Authors: | JIN Jian-zhong |
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| Institution: | JIN Jian-zhong(College of Biological and Environmental Engineering,Zhejiang Shuren University,Hangzhou 310015,China) |
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| Abstract: | Objective:To extract volatile oil of Perilla frutescens leaves by supercritical CO2 fluid,and then analyze the chemical components of volatile oil by GC-MS.Methods:Single factor and orthogonal design were applied for optimization of supercritical CO2 fluid extraction conditions extracting volatile oils of Perilla frutescens leaves.The effects of extraction pressure,extraction temperation,extraction time and carbon dioxide flow rate on the yield were investigated.The components and their contents in the vola... |
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| Keywords: | Perilla frutescens leaves volatile oil supercritical fluid extraction GC-MS orthogonal experiment design |
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