| 抗氧化活性藻蓝色素与牛血清白蛋白的相互作用 |
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| 引用本文： | 陈英杰,刘少芳,陈华新,李富超,秦松.抗氧化活性藻蓝色素与牛血清白蛋白的相互作用[J].药物分析杂志,2011(1). |
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| 作者姓名： | 陈英杰 刘少芳 陈华新 李富超 秦松 |
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| 作者单位： | 中国科学院海洋研究所实验海洋生物学重点实验室;中国科学院研究生院; |
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| 基金项目： | 国家海洋863重点项目(2006AA090303); 中科院知识创新工程重要方向性项目(KZCX2-YW-209,KZCX2-YW-216); 山东省基金项目(ZR2009EQ003) |
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| 摘 要： | 目的:进一步验证藻蓝色素(phycocyanobilin,PCB)的抗氧化作用,并研究藻蓝色素与牛血清白蛋白(bovine serum albu-min,BSA)之间的相互结合反应。方法:通过1,1-二苯基-2-苦基肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)清除法验证PCB的抗氧化作用。用荧光光谱法、分光光度法研究了PCB与BSA的相互结合反应。结果:PCB对DPPH自由基的清除作用呈现一定的量效关系。随着PCB浓度的增加,BSA的荧光强度有规律地降低且呈良好的线性关系。结论:抗氧化活性PCB能够有效地淬灭BSA的内源荧光,该猝灭作用属于静态荧光猝灭作用,反应的结合常数K=1.22×106L.mol-1,结合位点数n=1.14,与PCB的结合基本不会引起BSA的构象变化。
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| 关 键 词： | 藻蓝色素(PCB) 牛血清白蛋白(BSA) 荧光淬灭 |
Interaction between antioxidant phycocyanobilin and bovine serum albumin |
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| CHEN Ying-jie,LIU Shao-fang,CHEN Hua-xin,LI Fu-chao,QIN Song.Interaction between antioxidant phycocyanobilin and bovine serum albumin[J].Chinese Journal of Pharmaceutical Analysis,2011(1). |
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| Authors: | CHEN Ying-jie LIU Shao-fang CHEN Hua-xin LI Fu-chao QIN Song |
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| Institution: | CHEN Ying-jie1,2,LIU Shao-fang1,CHEN Hua-xin1,LI Fu-chao1,QIN Song1(1.Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China,2.Graduate University of Chinese Academy of Sciences,Beijing 100049,China) |
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| Abstract: | Objective:To confirm the antioxidant activity of phycocyanobilin(PCB) and study the interaction between PCB and bovine serum albumin(BSA) in aqueous solution.Methods:The antioxidant of PCB was evaluated with DPPH method.And the interaction between PCB and BSA was measured by fluorescence and UV-Vis absorption spectrometries.Results:DPPH radicals scavenging activity of PCB related to its concentration.The fluorescence intensity of BSA decreased with the increasing of the concentration of PCB regularly,which ... |
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| Keywords: | phycocyanobilin(PCB) bovine serum albumin(BSA) fluorescence quenching |
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