| HPLC-MS法分析不同提取方法黄芪三七配伍的化学成分变化 |
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| 引用本文： | 张靖,徐文,吴钉红,朱大元,卢传坚,丘小惠.HPLC-MS法分析不同提取方法黄芪三七配伍的化学成分变化[J].药物分析杂志,2011(6). |
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| 作者姓名： | 张靖 徐文 吴钉红 朱大元 卢传坚 丘小惠 |
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| 作者单位： | 广州中医药大学第二临床医学院;中国科学院上海药物研究所; |
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| 基金项目： | 2010年广东省中医院“中医特色与优势临床专项(第二批)”; 2009年广东省科技计划项目(2009A030100006) |
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| 摘 要： | 目的:研究采用两种不同提取方法时,黄芪和三七配伍前后化学成分的变化。方法:用HPLC法对单药及两药的水提醇沉法和醇提法提取液进行检测和比较分析,并结合LC-MS提供的分子量信息,推测产生变化的化学成分的结构。结果:黄芪和三七的水提醇沉法与醇提法的提取液相比,化学成分人参皂苷Rh1和Rg2的含量明显增加,且人参皂苷Rh4、三七皂苷Fe及三七皂苷T5等化学成分仅在水提醇沉提取液有检出;另有4个化学成分峰仅存在于醇提法的低极性部位,而在水提醇沉提取液中未检出。结论:单药及两药的提取液比较并没有新成分产生,仅在化学成分的含量上存在差异。而采用不同提取方法,黄芪和三七配伍的化学成分溶出种类及含量均有明显差别,尤其对三七化学成分的溶出影响较大。
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| 关 键 词： | 黄芪 三七 化学成分 高效液相色谱 质谱 生产工艺 |
HPLC study on chemical component changes in compatibility of Astragali Radix with Notoginseng Radix et Rhizoma by different extraction |
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| ZHANG Jing,XU Wen,WU Ding-hong,ZHU Da-yuan,LU Chuan-jian,QIU Xiao-hui.HPLC study on chemical component changes in compatibility of Astragali Radix with Notoginseng Radix et Rhizoma by different extraction[J].Chinese Journal of Pharmaceutical Analysis,2011(6). |
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| Authors: | ZHANG Jing XU Wen WU Ding-hong ZHU Da-yuan LU Chuan-jian QIU Xiao-hui |
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| Institution: | ZHANG Jing1,XU Wen1,WU Ding-hong1,ZHU Da-yuan2,LU Chuan-jian1,QIU Xiao-hui1(1.The Second Affialiated Clinical College,Guangzhou University of TCM,Guangzhou 510006,China,2.Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 211203,China) |
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| Abstract: | Objective:To explore the chemical constituents changes in compatibility of Astragali Radix(AR) with Notoginseng Radix et Rhizoma(NRR) by two different Extraction.Methods:HPLC was used to determine and analyze the peak changes of single and co-decoction by water extraction with alcohol precipitation and by 80% alcohol extraction,along with molecular information supporting by LC-MS,in order to speculate the changing components.Results:The content of ginsenoside Rh1 and Rg2 were increased in the water extract,... |
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| Keywords: | astragali radix notoginseng radix et rhizoma chemical component HPLC productive technology |
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