| 乙肝病毒标志物与PCR-HBV定量水平的关系 |
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| 引用本文： | 孙长义,王山梅.乙肝病毒标志物与PCR-HBV定量水平的关系[J].中国公共卫生,2002,18(12):1480-1481. |
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| 作者姓名： | 孙长义 王山梅 |
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| 作者单位： | 河南省人民医院检验科,郑州,450003 |
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| 摘 要： | 目的：研究乙型肝炎病毒标志物定量检测结果与其HBV－DNA含量的临床关系。方法：采用时间分辨免疫定量和荧光定量－PCR技术，对HBV－M和HBV－DNA含量进行检测。结果：在HBsAg/HBeAg/HBcAb,HBsAg/HBeAb/HBcAb,HBeAg/IC及单纯HBcAb阳性的4个组中，HBV－DNA阳性率分别是97．17％，30．56％，100％和25％；在HBsAg，HBeAg，HBeAb、HBcAb定量检测高、低值两组HBV－DNA含量对比中，HBV－DNA阳性率分别为76．92％，58．14％；100％，97．26％；20．00％，11．42％；64．62％和27．63％。结论：HBV－DNA比HBV－M更能及时准确反映乙型肝炎病毒感染的病程情况。
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| 关 键 词： | 乙型肝炎病毒 聚合酶链反应 DNA 免疫酶技术 |
| 文章编号： | 1001-0580(2002)12-1480-02 |
| 修稿时间： | 2002年5月2日 |
Preliminary Study on Relationships Between HBV Immunological Marker and HBV - DNA Concentrations |
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| SUN Chang-yi,WANG Shan-mei.Preliminary Study on Relationships Between HBV Immunological Marker and HBV - DNA Concentrations[J].Chinese Journal of Public Health,2002,18(12):1480-1481. |
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| Authors: | SUN Chang-yi WANG Shan-mei |
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| Institution: | SUN Chang-yi,WANG Shan-mei.Department of Clinical Laboratory,the People's Hospital of Henan Province |
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| Abstract: | ObjectiveTo investigate the relationships between HBV infected patients' immunological marker and HBV-DNA concentrations.MethodsTRFIA and FQ-PCR methods were applied in the analysis of HBV immunological marker and HBV-DNA concentrations in 216 HBV infected patients.ResultsIn patients with HBsAg+/HBeAg+/HBcAb+,HBsAg+/HBeAb+/HBcAb+,HBeAg+/IC+,and HBcAb+,the HBV-DNA positive rate were 97.17%,30.56%,100%,25%.In the high and low titer groups about HBsAg+,HBeAg+,HBeAb+,and HBcAb+,the HBV-DNA positive rate were 76.92%,58.14%;100%,97.26%;20.00%,11.42%;64.62%,27.63%.ConclusionHBV-DNA was more rapid and exact than HBV immunological marker to reflect the HBV infected patients. |
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| Keywords: | hepatitis B virus polymerase chain reaction DNA immunoassay |
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