磷脂酰肌醇3激酶信号通路影响细胞凋亡在肺腺癌A549细胞群集耐药中的作用

目的探讨PI3K信号通路在肿瘤群集耐药中的作用.方法采用旋转培养瓶结合台式水浴恒温振荡器旋转培养的方法,进行肺腺癌A.549细胞体外立体培养,通过Western blot、原位细胞凋亡检测、四唑盐(MTT)比色法及血球计数器计数法检测Akt、Bcl-2、Bad的表达、细胞凋亡率及对阿霉素(ADM)敏感性.结果①立体培养细胞存在Akt的高表达;②同平面培养比较,立体培养细胞存在Bcl-2高表达,Bad低表达,细胞凋亡率低,对ADM敏感性低的特征,阻断PI3K信号通路后,两者差异明显缩小.结论PI3K信号通路可通过抑制细胞凋亡在肺腺癌A549细胞群集耐药中发挥作用,阻断PI3K信号通路可能逆转该耐药现象.

Effects of PI3K pathway on multicellular resistance of lung adenocarcinoma A549 cells via affecting cell apoptosis

Objective To investigate the roles of PI3K pathway in multicellular resistance of lung adenocarcinoma A549 cells. Methods Three dimensional culture of A549 cells was conducted using rotatable culture bottle and desk top water bath shaker. Western blot, in situ cell apoptosis detection, MTT, and blood cell counter were employed to detect the expressions of Akt, Bcl 2, and Bad, the apoptotic rate, and the susceptibility of apoptosis to adriamycin (ADM) of A549 cells under different culture conditions. Results Compared with cells cultured in two dimensional culture system (2 D system), higher Akt and Bcl 2 expressions, lower Bad expression and apoptotic rate, and decreased susceptibility to ADM were found in cells cultured in three dimensional culture system (3 D system). The differences diminished significantly after blocking the PI3K pathway. Conclusion PI3K pathway has effect on the multicellular resistance of lung adenocarcinoma A549 cells via inhibiting cell apoptosis. Blocking this pathway may revert the resistance.