HBV-DNA定量与HBV模式及肝功损害指标定量关系研究

目的探讨HBV-DNA定量与HBV模式及肝功损害指标定量关系。方法随机抽选369例乙肝或疑似乙肝病毒感染者,荧光定量聚合酶链反应(PCR)对患者血清HBV-DNA定量,酶联免疫吸附法测定乙型肝炎血清标志物模式(HBV-M),采用全自动生化仪进行天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、γ-谷氨酰基转移酶(γ-GPT)、总胆红素(TBIL)等肝功指标含量的检测。结果 HBV模式下HBs Ag+HBe Ag+HBc Ab(大三阳)患者的血清HBV-DNA阳性检出率(93.8%)及平均含量对数值(7.31±1.23)明显高于HBs Ag+HBe Ab+HBc Ab(小三阳)患者(P0.05);HBs Ag+HBe Ab+HBc Ab(小三阳)患者的血清HBV-DNA阳性检出率(41.0%)及平均含量对数值(5.03±1.31)低于HBs Ag+HBc Ab患者(P0.05),但均显著高于其他各HBV-M模式(P0.05)。HBV-DNA定量与AST、ALT等肝功损害指标呈正相关性(r=0.235,P=0.014;r=0.254,P=0.009)。结论 HBV-DNA定量与HBV-M模式关系密切,与ALT、AST呈正相关性,定期检测HBV-M和HBV-DNA能全面了解HBV感染、复制以及传染性状态。

Study on quantitative relationship between quantification of HBV-DNA and HBV model or liver damage index

Objective To explore the quantitative relationship between quantification of HBV- DNA and HBV model or liver damage indexes. Methods A total of 369 cases of hepatitis B or suspected infection of hepatitis B virus were randomly selected for this study,fluorescence quantitative polymerase chain reaction（ PCR） had been applied for quantified determination of serum HBV- DNA,enzyme linked immunosorbent assay was used to determine the serum markers of hepatitis B model（ HBV- M）,automatic biochemical analyzer was used to detect the levels of liver function indexes including aspartate aminotransferase（ AST）,alanine aminotransferase（ ALT）,alkaline phosphatase（ ALP）,gamma glutamyltransferase（ γ- GPT） and total bilirubin（ TBIL）. Results Under HBV model,HBs Ag ＋,HBe Ag ＋ and HBc Ab in serum of patients with HBV- DNA positive rate of 93. 8% and the average content（ 7. 31 ± 1. 23） were significantly higher than those of numerical HBs Ag ＋,HBe Ab ＋ and HBc Ab（ P〈0. 05）; the positive rate（ 40%） of HBs Ag ＋,HBe Ab ＋ and HBc Ab in serum of patients with HBV- DNA and the average content of this value（ 5. 03 ± 1. 31） were significantly lower than those with HBs Ag ＋ and HBc Ab（ P〈0. 05）,but it was significantly higher than that of other HBV- M model（ P〈0. 05）. The content of HBV- DNA showed a positive correlation with ALT and AST（ r = 0. 235,P = 0. 014; r = 0. 254,P = 0. 009）. Conclusion The quantitative determination of HBV- DNA is closely associated with HBV- M,it is positively correlated with ALT and AST,regular detection of HBV- M and HBV- DNA can comprehensively understand the status of HBV infection,replication and communicability.