人胎肝细胞体外感染HCV的定性研究

目的：通过定性研究探讨人胎肝细胞在体外与丙型肝炎病毒（ＨＣＶ）阳性血清共培养２４ｈ是否能感染ＨＣＶ。方法：人胎肝细胞在体外与ＨＣＶ阳性血清共培养２４ｈ后，采用原位ＲＴ－ＰＣＲ技术对其进行ＨＣＶ定性检测。结果：通过原位ＲＴ－ＰＣＲ技术检测，发现人胎肝细胞内ＨＣＶ正、负链ＲＮＡ均为阳性。此外，将人胎肝细胞在体外与ＨＣＶ阳性血清共培养２４ｈ并经多次洗涤后继续培养的上清液加入到新鲜分离的正常人胎肝细胞培养

Qualitative Research of Human Fetal Hepatocytes Infected with Hepatitis C Virus in Vitro

Aim: Qualitative researches were carried out to confirm whether human fetal hepatocytes (HFH) were infected with hepatitis c viruses (HCV) after co-culturing with HCV positive serum in vitro for 24 hours. Methods: The HFH co-cultured with HCV positive serum were carried out the qualitative researches by means of in situ RT-PCR technique to determinate whether there were HCV RNA in the cells. Results: It was found that the plus and minus strands of HCV RNA in the cells were all positive, after examinated by in situ RT-PCR technique. Moreover, the culture supernatant of HFH co-culturing with HCV positive serum for 24 hours and through washing for many times was added into the culture medium of HFH separated freshly and continued to culture, the same results mentioned above might be arrived at. Conclusions; HFH may be infected with HCV in the circumstances of culture in vitro aid support HCV replicating in the cells to produce contagious HCV particles to secrete into the culture supernatant arc und HFH.