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| 白介素8B型受体在大鼠脑垂体中的分布和表达 | |
| 引用本文： | 韩雪峰,张万会,陈健康,邓艳春,胡玉珍. 白介素8B型受体在大鼠脑垂体中的分布和表达[J]. 细胞与分子免疫学杂志, 2001, 17(2): 128-130 |
| 作者姓名： | 韩雪峰 张万会 陈健康 邓艳春 胡玉珍 |
| 作者单位： | 1. 第四军医大学生理学教研室 , 陕西西安 710032 2. 第四军医大学实验教学中心 , 陕西西安 710032 3. 第四军医大学西京医院神经内科 , 陕西西安 710032 |
| 摘 要： | 目的：探讨白介素8B型受体（interluekine-8 receptorB,IL-8RB)在大鼠脑垂体中的分布和表达。方法用免疫组化法，观察该受体在垂体中的分布。提取组织和原代培养细胞的总RNA，用RT－PCR检测该受体mRNA的表达。结果IL－8RB细胞主分布垂体前叶。测序结果证实，PCR产物为IL－8RBmRNA。结论首次证实在大鼠脑垂体中有IL－88型受体的表达及其分布区。 |
| 关 键 词： | 脑垂体 IL－8B型受体 RT－PCR 克隆 免疫组化染色 原代细胞培养 |
| 文章编号： | 1007-8738(2001)02-128-03 |
Distribution andexpression of interluekin-8 receptor B in the rat pituitary |
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| HAN Xue feng,ZHANG Wan hui,CHEN Jian kang,DENG Yan chun,HU Yu zhen. Distribution andexpression of interluekin-8 receptor B in the rat pituitary[J]. Chinese journal of cellular and molecular immunology, 2001, 17(2): 128-130 | |
| Authors: | HAN Xue feng ZHANG Wan hui CHEN Jian kang DENG Yan chun HU Yu zhen |
| Affiliation: | HAN Xue feng1,ZHANG Wan hui1,CHEN Jian kang2,DENG Yan chun3,HU Yu zhen11Department of Physiology,2Center of Experiment Technology,3Department of Neurology,Xijing Hospital,Forth Military Medical University,Xi'an 710032,Shannxi Province,Chi |
| Abstract: | Aim To explore the distribution and expression of interluekin 8 receptor B (IL 8RB) in the rat pituitary. Methods Distribution of IL 8RB and its mRNA expression in rat pituitary tissue were detected by RT PCR and immunohistochemical staining respectively. Results IL 8RB distributed mainly in the anterior pituitary. The PCR product is identical with rat IL 8RB. Conclusion It firstly demonstrates that there are IL 8RB distributive position and expression in rat pituitary. |
| Keywords: | pituitary interluekin 8 receptor B RT PCR cloning immunohistochemical staining primary cell culture |
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