Evaluation of two new chromogenic media for detection ofSalmonella in stools |
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| Authors: | I. Monnery A. M. Freydiere C. Baron A. M. Rousset S. Tigaud M. Boude-Chevalier H. de Montclos Y. Gille |
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| Affiliation: | (1) Laboratoire de Microbiologie, Hôpital de l'Antiquaille, Institut Pasteur de Lyon, 69321 Lyon Cedex 5, France;(2) Laboratoire de Microbiologie, Hôpital de la Croix-Rousse, Grande Rue de la Croix-Rousse, 69004 Lyon, France;(3) Laboratoire de Microbiologie, Institut Pasteur de Lyon, Avenue Tony Garnier, 69365 Lyon Cedex 07, France |
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| Abstract: | A clinical collaborative study was conducted to compare two new chromogenic agar media, Rambach agar and theSalmonella Detection and Identification Medium (SMID) (bioMérieux, France), with two conventional media, Salmonella-Shigella agar and Hektoen agar. Thirty-nineSalmonella strains involving 14 serotypes were isolated from 1,454 stool specimens. After enrichment in a selective broth, 100 % sensitivity was obtained with each medium. The SMID and Rambach agars are considerably more specific than the conventional media. Although SMID agar detects allSalmonella serotypes, it is not as specific as Rambach agar, which requires a complementary test (C8 esterase test) to detect all serotypes. |
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