| 辣根过氧化酶标记神经节苷脂免疫测定法检测霍乱肠毒素、类毒素和破伤风毒素、类毒素 |
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| 引用本文： | 陈哲生. 辣根过氧化酶标记神经节苷脂免疫测定法检测霍乱肠毒素、类毒素和破伤风毒素、类毒素[J]. 中国免疫学杂志, 1986, 0(4) |
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| 作者姓名： | 陈哲生 |
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| 作者单位： | 首都医学院微生物学教研室 北京 |
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| 摘 要： | Heymlngen(1974)报道了神经节苷脂(Gauglloslde)能结合破伤风毒素,但仅能延缓其毒性,不久,又报导霍乱肠毒素也能与Gm结合,并被灭活。随之许多学者,利用神经节苷脂建立了各种体外测定细菌毒素的方法,如琼脂双扩散,Gm1神经节苷脂酶联免疫吸附法等。
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Identification of Cholerae toxin (or toxoid) and Tetanus toxin (or toxoid) by means of a ganglioside linked horse radish peroxidase Immunoassay |
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| Chen Zhe-Sheng. Identification of Cholerae toxin (or toxoid) and Tetanus toxin (or toxoid) by means of a ganglioside linked horse radish peroxidase Immunoassay[J]. Chinese Journal of Immunology, 1986, 0(4) |
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| Authors: | Chen Zhe-Sheng |
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| Affiliation: | Department of Microbiocolgy Capital Institute of Medecine |
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| Abstract: | This paper describes the elaboration of the use of HRP labelled Gangliosides as second antibody to the tentative identification of Cholera enterotoxin(toxoid)and Tetanus toxin(toxoid).The method is called Enzyme linked Ganglioside Immunesorbent assay (ELGISA).The sensitivity of this was campared with those of Ouchlerlony double diffusion method,VCS and TLA,but found to be more sensitive. |
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| Keywords: | VCS Vapour condensation on surface TLA Thin layer in gel immunoassay. |
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