Synthesis and evaluation of anti‐inflammatory properties of silver nanoparticle suspensions in experimental colitis in mice |
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| Authors: | Anna Chmielowiec‐Korzeniowska Jolanta Pulit‐Prociak Magdalena ?miech Radzis?aw Kordek Leszek Tymczyna Marcin Banach Jakub Fichna |
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| Institution: | 1. Department of Animal Hygiene and Environment, Faculty of Biology and Animal Breeding, University of Agriculture in Lublin, Lublin, Poland;2. Institute of Chemistry and Inorganic Technology, Cracow University of Technology, Cracow, Poland;3. Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, Poland;4. Department of Pathology, Faculty of Medicine, Medical University of Lodz, Lodz, Poland;5. Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, Poland |
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| Abstract: | The aim of our study was to investigate the effect of newly developed silver nanoparticle aqueous suspensions NanoAg1 and NanoAg2 in the mouse models mimicking ulcerative colitis and Crohn's disease. NanoAg1 and NanoAg2 were synthesized in aqueous medium with the involvement of tannic acid. To elucidate their anti‐inflammatory activity, semi‐chronic mouse models of inflammation induced by dextrane sulfate sodium addition to drinking water and intracolonic (i.c.) administration of 2,4,6‐trinitrobenzenesulfonic acid were used. NanoAg1 and NanoAg2 (500 mg/dm3, 100 μl/animal, i.c., once daily) significantly ameliorated colitis in dextrane sulfate sodium‐ and 2,4,6‐trinitrobenzenesulfonic acid‐induced mouse models of colonic inflammation, as indicated by reduced macroscopic, ulcer and microscopic scores. The anti‐inflammatory effect was dependent on the shape and diameter of silver nanoparticles, as indicated by weaker effect of NanoAg1 than NanoAg2. In addition, administration of NanoAg2, but not NanoAg1, modulated colonic microbiota, as indicated by reduced number of Escherichia coli and Clostridium perfringens, and increased number of Lactobacillus sp. Summarizing, NanoAg1 and NanoAg2 after administered i.c. effectively alleviate colitis in experimental models of ulcerative colitis and Crohn's disease in mice. Therefore, NanoAg1 and NanoAg2 administered i.c. have the potential to become valuable agents for the treatment of inflammatory bowel diseases. |
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| Keywords: | Crohn's disease inflammatory bowel disease microbiota silver nanoparticles ulcerative colitis |
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