Solasodine inhibits human colorectal cancer cells through suppression of the AKT/glycogen synthase kinase‐3β/β‐catenin pathway |
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| Authors: | Xu Chen Jian Wu Shan Jiang Hai‐yan Peng Xi Zou Jia‐yun Liu Da‐peng Wu Tao Gong Ming‐hao Qi Tian Xue Shen‐lin Liu Hui Cai |
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| Affiliation: | 1. The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, China;2. Jiangsu Province Hospital of TCM, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, china;3. Division of Membrane Dynamics, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe, Japan;4. Nanjing Hospital of Traditional Chinese Medicine, Nanjing, China;5. Department of Integrated Traditional and Western Medicine, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China |
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| Abstract: | Solasodine is a main active component isolated from Solanum incanum L. that performs a wide range of functions containing anti‐oxidant, anti‐infection, and neurogenesis promotion. In this study, we explored the influence of solasodine on three types of human colorectal cancer (CRC) cell lines. The results show that solasodine prohibited CRC cell proliferation dose‐ and time‐dependently and impeded CRC cell motility by downregulating MMPs. Solasodine was also found to fuel caspase‐cascade reaction and increase the ratio between Bax and Bcl‐2 so as to induce CRC cell apoptosis. When cells were pretreated with AKT activator (insulin‐like growth factor‐1) followed by solasodine, the solasodine‐induced apoptosis was partially abrogated by insulin‐like growth factor‐1. Moreover, solasodine hindered tumor development and stimulated similar mechanisms in vivo. In general, our study provides the first evidence that solasodine has a suppressive effect on CRC cells and that this agent may be a novel therapeutic drug for CRC treatment. |
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| Keywords: | Apoptosis colorectal cancer metastasis solasodine β ‐Catenin |
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