光动力学疗法过程中小鼠皮肤蛋白质谱变化的研究

目的 寻找皮肤对血卟啉单甲醚光动力学疗法(hematoporphyrin monomethyl ether mediated photodyrnamic therapy,HMME-PDT)应答的蛋白质分子标志物.方法 雌性BALB/c小鼠尾静脉注射血卟啉单甲醚(hematoporphyrin monomethyl ether,HMME)10 mg/kg后即刻以532 nm的激光对其皮肤进行照射,功率密度50 mW/cm2,光斑直径1 cm,照光时间20 min;同时设立单纯照光组、单纯光敏剂组以及空白对照组.术后24 h从照射局部皮肤取材,以裂解液提取皮肤蛋白,然后用蛋白质芯片(WCX2)和SELDI蛋白质芯片阅读机检测小鼠皮肤差异蛋白质的表达.结果 HMME-PDT能够明显改变照射局部皮肤蛋白质谱的表达,在质荷比为相对分子质量(Mr)4949～18 828之间,共有7个蛋白质峰的强度发生了显著改变,其中2个蛋白质表达上调,另外5个蛋白质表达下调.单独照光组在Mrl4 927处有1个蛋白质的表达下调,与PDT处理组在该点的变化一致,且与正常对照相比有显著差异.结论 PDT照射能显著改变小鼠皮肤蛋白质谱的表达,单纯照光也能引起个别蛋白的表达发生改变,并且与PDT在该点的表达趋势一致,提示这些差异蛋白质的表达与皮肤对PDT所产生的氧化应激或照光引起的光热效应的应答机制有关.

Hematoporphyrin Monomethyl Ether-mediated Photodynamic Therapy on Expression of Mice Skin Proteins:a Mass Spectometry Study

Objective To elucidate the specific biomarker of irradiated mice skin to hematoporphyrin monomethyl ether(HMME) mediated photodynamic therapy(HMME-PDT). Methods Female BALB/c mice were irradiated by 532 nm laser at power density of 50 mW/cm2 for 20 min after intravenous injection of 10 mg/kg HMME immediately. The irradiated skin tissue was harvested and then homogenized in lysis buffer, soluble protein was quantitated and used for surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry...