| IgA肾病患者血清和肾组织内巨细胞病毒DNA的检测 |
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| 引用本文： | 刘志红,黎磊石,周虹.IgA肾病患者血清和肾组织内巨细胞病毒DNA的检测[J].医学研究生学报,1991(4). |
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| 作者姓名： | 刘志红 黎磊石 周虹 |
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| 作者单位： | 金陵医院全军肾脏病中心，金陵医院全军肾脏病中心，金陵医院全军肾脏病中心 |
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| 摘 要： | 本文选用多聚酶链反应(PCR)技术,对10例表现为肉眼血尿的 IgAN.10例非肉眼血尿IgAN.10例系膜增殖性肾炎和10例膜性肾病患者血清中 CMV-DNA 进行了检测,同时应用原位分子杂交技术对 IgAN 患者肾组织中 CMV-DNA 进行了观察.发现肉眼血尿型 IgAN CMV感染率明显高于非肉眼血尿型IgAN 及其它原发性肾小球肾炎,表明 CMV 感染与 IgAN 肉眼血尿的发作关系密切.IgAN 肾组织中 CMV-DNA 的检出率明显低于血清中,提示 CMV 感染只是使 IgAN 病情加剧的一个触发因素,而并非致病因素。
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| 关 键 词： | 多聚酶链反应 巨细胞病毒 IgA 肾病 原位杂交 |
Study of the cytomegalovirus-DNA in serum and renal tissue in patients with IgA nephropathy |
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| Liu Zhihong,et al.Study of the cytomegalovirus-DNA in serum and renal tissue in patients with IgA nephropathy[J].Bulletin of Medical Postgraduate,1991(4). |
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| Authors: | Liu Zhihong |
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| Institution: | Department of Nephrology |
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| Abstract: | Cytomegalovirus(CMV)has been suspected to be involved in the pathogenesis of IgA nephropathy(IgAN),In this study,IgAN both with and without recurrent gross hematuria(GH),cases of MsPGN,MN were in- cluded,10 cases in each group.Serum were obtained and analyzed for the presence of CMV-DNA with polymerase chain reaction.Renal biopsies of the 20 cases of IgAN were also analyzed for the presence of CMV-DNA with in situ hybridization.The positive rates of serum CMV-DNA were 7/10 in IgAN (GH),2/10 in IgAN(non-GH),1/10 in MsPGN and 2/10 in MN.It was much higher in IgAN(GH) than in the other groups.There was only 1 case showed CMV-DNA in renal tissue in IgAN(GH)and IgAN(non-GH) respectively. The high frequency of CMV-DNA present in the serum but not in the renal tissue of IgAN implicated that there was a close association but not etiological relationship between them. |
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| Keywords: | polemerase chain reaction cytomegalovirus IgA nephropathy in situ hybridization |
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