仙草消毒液杀灭微生物效果与毒性的试验观察

仙草消毒液为含 3 0 %乙醇的植物消毒剂 ,其中包括五味子、细辛、丁香等成分 ,p H6.0 1。为了解其杀灭微生物效果及其毒性 ,进行了载体定量杀菌试验及毒性试验。结果 ,1∶ 1的水稀释液对金黄色葡萄球菌作用2分钟 ,1∶ 2的稀释液对金黄色葡萄球菌作用 8分钟 ,以其原液对白色念珠菌作用 2 min,平均杀灭率均大于99.90 %。小牛血清对其杀灭效果有影响 ,原液经 5 4℃存放 14天 ,杀菌效果无明显变化。以其原液对雌、雄小鼠经口 LD50 >5 0 0 0 mg/Kg,对大耳白兔一次眼、皮肤刺激试验平均积分指数为 0 ,微核试验阴性 ,蓄积毒性试验为弱蓄积毒性

EXAMINATION OF GERMICIDAL EFFICACY AND TOXICIY OF XIANCAO DISINFECTANT

Xiancao disinfectant is the herbal disinfectant including 30% ethyl alcohol? schisandra? asarum? clove etc. pH 6.01. In order to know the germicidal efficacy and doxicity, carrier quantitative germicidal efficacy test and animical toxicity test were carried out. The resulut showed that the average killing rates of Staphylococus aureus exposed to its 1∶1 water diluted solution for 2 min? 1∶2 water diluted solution for 8 min, Candida albicans exposed to its stock solution for 2 min attained 99.90%. The organic substance had certain influence on its bactericial efficacy. After storing at 54℃ for 14 days, its germicidal efficacy had no obvious change. Toxicity test indicate that its LD 50 >5000mg/Kg in both female and male mice and its stock solution did not cause abnormal reaction of rabbit eye and male mice and its stock solution did not cause abnormal reaction of rabbit eye and skin. The mouse bone marrow micronucleus test was negative and the accumulation toxicity test was weak.