Measurement of intracellular calcium levels by the fluorescent Ca(2+) indicator Calcium-Green |
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| Authors: | Silei V Fabrizi C Venturini G Tagliavini F Salmona M Bugiani O Lauro G M |
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| Affiliation: | Dipartimento di Biologia, Università di Roma Tre, Viale Marconi 446, 00146, Rome, Italy. |
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| Abstract: | The fluorescent calcium-sensitive indicators, such as the Calcium Green-1, allow one to detect small calcium transients at low indicator concentrations. The protocol reported here is a rapid and sensitive method that facilitates the measurement of intracellular free-calcium in cell suspensions. Using this assay, we were able to detect and quantify the variations in intracellular calcium concentration during microglial cell activation induced by the fragment peptides beta25-35 and PrP106-126. |
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