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| 旋毛虫新生幼虫cDNA文库的免疫筛选 | |
| 引用本文： | 吴秀萍,付宝权,刘明远,张亚兰,原丽红,李莲瑞,卢强,Pascal Boireau. 旋毛虫新生幼虫cDNA文库的免疫筛选[J]. 中国寄生虫学与寄生虫病杂志, 2004, 22(4): 235-239 |
| 作者姓名： | 吴秀萍 付宝权 刘明远 张亚兰 原丽红 李莲瑞 卢强 Pascal Boireau |
| 作者单位： | 1. 解放军军需大学军事兽医系,长春,130062 2. 解放军军需大学军事兽医系,长春,130062;法国食品卫生安全局,巴黎,94703 3. 法国食品卫生安全局,巴黎,94703 |
| 基金项目： | 国家自然科学基金 (No 30 1 70 70 9)，国际科学基金(B/ 352 5 1)~~ |
| 摘 要： | 目的 获取具有抗原性的旋毛虫新生幼虫基因。 方法 以旋毛虫感染猪血清为抗体探针,对旋毛虫新生幼虫cDNA文库进行免疫筛选,将获得的阳性克隆进行测序及分析。结果 从45×105旋毛虫新生幼虫cDNA文库重组子中共获得158个阳性克隆。序列分析结果表明,其中有36个新的cDNA序列,已报道的序列有5个,有12个序列无开放阅读框架(ORF),与旋毛虫线粒体基因组DNA同源。 结论 获得具有抗原性的旋毛虫抗原编码cDNA分子 |
| 关 键 词： | 旋毛虫 DNA序列分析 基因文库 免疫筛选 |
| 文章编号： | 1000-7423(2004)-01-0235-05 |
| 修稿时间： | 2003-07-21 |
Immunoscreening of Newborn Larvae cDNA Library of Trichinella spiralis |
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| WU Xiu-ping,FU Bao-quan,LIU Ming-yuan,ZHANG Ya-lan,YUAN Li-hong,LI Lian-rui,LU Qiang,Pascal Boireau. Immunoscreening of Newborn Larvae cDNA Library of Trichinella spiralis[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2004, 22(4): 235-239 | |
| Authors: | WU Xiu-ping FU Bao-quan LIU Ming-yuan ZHANG Ya-lan YUAN Li-hong LI Lian-rui LU Qiang Pascal Boireau |
| Affiliation: | Faculty of Military Veterinary, Quartermaster University of PLA, Changchun 130062, China. |
| Abstract: | OBJECTIVE: To obtain antigenic genes encoding newborn larvae antigens of Trichinella spiralis. METHODS: Newborn larvae (NBL) cDNA library of Trichinella spiralis was screened using the infected swine serum and positive clones were sequenced and analysed. RESULTS: 158 positive clones were obtained from 4.5 x 10(5) recombinant clones by immunoscreening. The sequence analysis of positive clones showed that there are 36 novel cDNAs, 5 reported cDNAs and 12 cDNAs without the ORF showing high similarity to the mitochondrial DNA of Trichinella spiralis. CONCLUSION: Some cDNAs encoding antigenic protein of newborn larvae of Trichinella spiralis were obtained. |
| Keywords: | Trichinella spiralis Sequence analysis DNA Gene library Immunoscreening |
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