四氯化碳对体外培养肝星状细胞钙浓度的影响

目的研究四氯化碳(CC l4)对肝星状细胞内游离Ca2 ([Ca2 ]i)的影响。方法用常规的细胞培养方法,每组加入5~15 mmol/L的CC l4处理细胞,分别作用2~8 h后,以F luo-3/AM进行荧光染料负载,用激光共聚焦显微镜观察不同剂量CC l4作用不同时间[Ca2 ]i的变化。结果CC l4在5~15 mmol/L范围内可明显增加肝星状细胞[Ca2 ]i,且[Ca2 ]i随CC l4剂量的增大而升高(P<0.05),作用2 h后,各组[Ca2 ]i均上升,与对照组比较差异有显著性(P<0.05),4 h后各组[Ca2 ]i继续升高;8 h后各组[Ca2 ]i维持在较高水平,各组分别与作用4 h比较差异均无显著性(P<0.05)。结论CC l4可能引起肝星状细胞[Ca2 ]i升高。

Effect of carbon tetrachloride on calcium concentration of hepatic stellate cells in vitro

Objective To study the effects of carbon tetrachloride(CCl_4)on intracellular calcium concentration([Ca~(2 )]i) of hepatic stellate cells(HSC).Methods Hepatic stellate cells were cultured and treated at the doses of 5-15 mmol/L CCl_4 for 2-8 hours respectively.After the cells were loaded with fluorescent indicator Fluo-3/AM, [Ca~(2 )]i levels in HSC were measured by laser scanning confocal microscopy(LSCM).Results At the doses of 5-15 mmol/L CCl_4,[Ca~(2 )]i levels in HSC were sharply increased(P<0.05) and related to the dosage of CCl_4.[Ca~(2 )]i levels in each group were increased(P<0.05) when HSCs were treated with CCl_4 for 2 hours and had significant differences with those of the control(P<0.05).[Ca~(2 )]i levels in HSC at 4 hours were continuously increased and remained at high stable levels at 8 hours.In comparison with [Ca~(2 )]i levels in HSC at the time of 4 hours,no significant difference was observed in other dose groups.Conclusion CCl_4 could cause the increase of [Ca~(2 )]i level of hepatic stellate cells.