中药复方912注射液对MODS动物模型氧代谢的实验研究

目的为探讨复方912注射液对多器官功能障碍综合征(multiple organ dysfunction syndrome,MODS)动物模型机体组织细胞氧代谢的影响。方法选择体质量20～25kg的1年期雄性健康小香猪32只,以标准大肠杆菌由右侧颈内静脉缓慢注入,于注入5h后制成MODS动物模型,采用随机数字表法将MODS动物模型随机分为912液治疗组和0.9%氯化钠注射液对照组。2组动物模型分别于治疗前及治疗后1、3、5、7h采用热稀释原理测定心输出量(cardiac output,CO),测定CO的同时由Swan-Ganz漂浮导管的肺动脉测孔抽取混合静脉血,由颈动脉鞘管抽取动脉血进行血气分析。将所得的数据及动物当时的血红蛋白和吸氧浓度(21%)输入监护仪计算出CaO2(arterial oxygen content,动脉氧含量)、氧输送量(oxygen delivery,DO2)、氧消耗量(oxygen consumption,VO2)、氧输送指数(oxygen delivery index,DO2I)、氧消耗指数(oxygen consumption index,VO2I)、氧摄取指数(oxygen extraction rate,O2Ext)。结果经过复方912液的治疗MODS动物模型DO2在接受治疗5、7h后较治疗前有明显改善,差异有统计学意义(P=0.036),VO2在治疗7h后较治疗前有明显改善,差异有统计学意义(P=0.028);DO2I、VO2I在治疗3、5、7h后较治疗前有明显改善,差异有统计学意义(P=0.031,P=0.027),而O2Ext在治疗后1h即较治疗前有显著改善,差异有统计学意义(P=0.033),治疗后5、7h较治疗后1、3h也有显著改善,差异有统计学意义(P=0.031)。结论复方912液可明显改善MODS动物模型氧代谢指标,为912液对MODS的进一步临床治疗研究提供了实验基础。

Effect of the Traditional Chinese Medicine Complex 912 Injection on Tissue Oxygenation in the Animal Model of Multiple Organ Dysfunction Syndrome

Objective To study the effect of the traditional Chinese medicine complex 912(Co 912) injection on the tissue oxygenation in the multiple organ dysfunction syndrome(MODS) animal model. Methods Totally 32 one-year-old male piglets with body weight 20~25 Kg were selected for this study. Escherichia coli(E. coli, 17 million/Kg) was injected into right jugular vein, and 5 hours later, all the animals developed MODS, and these MODS model piglets were randomly divided into the following groups: Co 912 injection treatment group; and normal saline control group. The tissue oxygenation-index(arterial oxygen content, CaO₂, oxygen delivery, DO₂, oxygen consumption, VO₂, oxygen delivery index, DO₂I, oxygen consumption index, VO₂I, oxygen extraction rate, O₂ Ext)were determined at 1, 3, 5, 7 h after treatment. Results DO₂ were remarkably better after 5 h or 7 h; VO₂ were remarkably better after 7 h; DO₂I, VO₂I were remarkably better;O₂ Ext was remarkably better after 1 h treatment with Co 912 injection than before treatment. Conclusion The tissue oxygenation-index were remarkably better after treatment with Co 912 injection after 3, 5, 7 h.