雄激素对大鼠阴茎海绵体平滑肌中兰尼碱受体1和电压门控钙离子通道1.3表达的影响研究

目的:研究兰尼碱受体1(RyR1)和电压门控钙离子通道1.3(CaV1.3)在去势大鼠阴茎海绵体平滑肌的表达,探讨其在去势后勃起功能障碍发生中的作用。方法:40只8周龄雄性SD大鼠随机均分成:假手术2周组(A组),假手术4周组(B组),去势2周组(C组),去势4周组(D组)。术后实验各组检测血清睾酮(T)水平,免疫组化及RT-PCR技术检测RyR1和CaV1.3在大鼠阴茎海绵体平滑肌中的表达。结果:血清T水平C组[(15.97±5.67)nmol/L]和D组[(2.03±1.57)nmol/L]分别较A组[(90.54±20.13)nmol/L]和B组[(120.35±30.32)nmol/L]显著下降(P<0.05)。RyR1、CaV1.3在各组大鼠阴茎海绵体平滑肌中均有表达,RyR1 mRNA相对表达量灰度比值C组(0.51±0.24)和D组(0.33±0.15)分别较A组(1.53±0.25)和B组(1.37±0.23)显著降低(P<0.05);CaV1.3 mRNA相对表达量灰度比值C组(0.50±0.12)和D组(0.32±0.07)分别较A组(1.33±0.05)和B组(1.25±0.03)显著降低(P<0.05)。RyR1蛋白积分光密度值(IA)C组(120.36±25.78)和D组(67.39±30.54)分别较A组(300.96±135.12)和B组(330.38±128.59)显著降低(P<0.05);CaV1.3蛋白IA C组(103.37±39.52)和D组(67.56±20.15)分别较A组(298.68±126.35)和B组(327.35±117.37)显著降低(P<0.05)。雄激素水平与RyR1、CaV1.3在阴茎海绵体平滑肌中的表达水平具有正相关性。结论:雄激素可能通过RyR1、CaV1.3的表达调控阴茎勃起功能。

Androgen Correlates with Expressions of Ryanodine Receptor 1 and Voltage-Gated Calcium Channel 1.3 in Rat Corpus Cavernosum Smooth Muscle

Objective: To study the expressions of ryanodine receptor 1(RyR1) and voltage-gated calcium channel 1.3(CaV1.3) in the corpus cavernosum smooth muscle of castrated rats and to investigate their role in androgen deficiency-related erectile dysfunction.Methods: Forty 8-week-old SD rats were equally randomized into Groups A(2-week sham-operation),B(4-week sham-operation),C(2-week castration),and D(4-week castration).After surgery,the levels of serum testosterone in different groups of rats were determined,and ...