| HIV-1母婴传播早期诊断检测方法应用 |
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| 引用本文： | 贺健梅,陈曦,李向忠,张国强,江洋,邹潇白,丁莉莎.HIV-1母婴传播早期诊断检测方法应用[J].中国自然医学杂志,2011(4). |
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| 作者姓名： | 贺健梅 陈曦 李向忠 张国强 江洋 邹潇白 丁莉莎 |
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| 作者单位： | 湖南省疾病预防控制中心; |
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| 基金项目： | 全球基金中国艾滋病防治项目(CHN-304-G03-H); 湖南省卫生厅科研计划项目(B2010-091) |
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| 摘 要： | 目的应用核酸扩增技术对母婴传播婴幼儿人类免疫缺陷病毒(HIV)感染进行早期诊断,为临床应用提供依据。方法对2009-2010年HIV阳性孕产妇活产的34例3~14个月龄婴幼儿收集足底干血斑样本进行HIV-1 DNA检测。随访18个月后进行血清HIV抗体检测比较。结果 34例婴幼儿中,检出HIV-1 DNA 9例,检出率26.5%。9例HIV-1 DNA阳性患儿均未使用过抗病毒治疗。18个月后随访,34例婴幼儿除1例失访,2例检出HIV-1 DNA婴儿已死亡(根据临床有机会性感染体征诊断为AIDS),7例检出HIV-1 DNA婴儿血清抗体阳性外,其他24例未检出HIV-1 DNA的婴儿其血清抗体为阴性。两种方法检测结果比较符合率为100.0%。结论核酸检测是有效的早期诊断方法,早期诊断有利于后续开展母婴阻断工作。
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| 关 键 词： | 母婴传播 HIV-1DNA检测 蛋白印迹试验 HIV抗体 干血斑样本 |
Early laboratory detection of HIV type 1 in infants |
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| HE Jian-mei,CHEN Xi,LI Xiang-zhong,ZHANG Guo-qiang,JIANG Yang,ZOU Xiao-bai,DING Li-sha Hunan Provincial Center for Disease Prevention , Control,Changsha,Hunan ,China.Early laboratory detection of HIV type 1 in infants[J].Chinese Journal of Natural Medicine,2011(4). |
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| Authors: | HE Jian-mei CHEN Xi LI Xiang-zhong ZHANG Guo-qiang JIANG Yang ZOU Xiao-bai DING Li-sha Hunan Provincial Center for Disease Prevention Control Changsha Hunan China |
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| Institution: | HE Jian-mei,CHEN Xi,LI Xiang-zhong,ZHANG Guo-qiang,JIANG Yang,ZOU Xiao-bai,DING Li-sha Hunan Provincial Center for Disease Prevention and Control,Changsha,Hunan 410005,China |
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| Abstract: | Objective To establish and evaluate polymerase chain reaction for the early detection of HIV type 1 in infants.Methods A total of 34 dried blood spot(DBS) samples were collected during 2009 and 2010 from 3-14 months infants of HIV positive mothers.All the specimens were tested by qualitative HIV-1 DNA polymerase chain reaction.HIV antibodies were tested with Western blot assay after 18 months following-up.Results HIV-1 DNA was positive in 9(26.5%) samples by HIV-1 DNA polymerase chain reaction.Of 9 HIV-1 DN... |
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| Keywords: | Mother-to-child transmission HIV-1 DNA polymerase chain reaction Western blot test HIV antibody Dried blood spot(DBS) sample |
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