| 福州市甲型H1N1流感病毒血凝素基因遗传特性研究 |
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| 引用本文： | 姚栩,陈智伟,张彩云,夏晓峰,杨建娜,郑能雄. 福州市甲型H1N1流感病毒血凝素基因遗传特性研究[J]. 中国自然医学杂志, 2011, 0(3) |
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| 作者姓名： | 姚栩 陈智伟 张彩云 夏晓峰 杨建娜 郑能雄 |
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| 作者单位： | 福州市疾病预防控制中心; |
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| 摘 要： | 目的探究福州市甲型H1N1流感病毒血凝素(hemagglutinin,HA)基因的遗传变异特点及种系进化分布,为研究甲型H1N1流感病毒进化、致病性和流行规律提供科学依据。方法采用MDCK细胞培养法和real-time PCR法进行流感病毒分离、鉴定。采用逆转录-聚合酶链反应扩增流感病毒血凝素基因,扩增产物经纯化后,进行血凝素基因HA区核苷酸序列测定并推导出其氨基酸序列,然后用DNASTAR MegAlign序列分析软件进行基因种系进化特性分析。结果福州市甲型H1N1流感病毒与参考株A/California/04/2009相比具有高度同源性,HA蛋白有4个位点氨基酸发生了替换,与猪流感代表株A/swine/Iowa/15/1930(H1N1)存在6个HA抗原决定簇位点变异。福州市甲型H1N1流感病毒株都有8个糖基化位点,其中6个位于HA1区,分离株HA蛋白不具有多碱基裂解位点,具有低致病性流感病毒特点。与美国流感病毒A/California/04/2009(H1N1)株共聚类为一个独立的小分支,进化关系最近,甲型H1N1流感病毒的HA源于古典型猪H1N1流感病毒,与A/swine/Iowa/1/1976(H1...
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| 关 键 词： | 甲型H1N1流感病毒 血凝素基因 序列 |
Genetic characterization of the hemagglutinin gene of 2009 H1N1 influenza viruses isolated in Fuzhou,Fujian |
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| YAO Xu,CHEN Zhi-wei,ZHANG Cai-yun,XIA Xiao-feng,YANG Jian-na,ZHENG Neng-xiong Fuzhou Center for Disease Control , Prevention,Fuzhou,Fujiang ,China. Genetic characterization of the hemagglutinin gene of 2009 H1N1 influenza viruses isolated in Fuzhou,Fujian[J]. Chinese Journal of Natural Medicine, 2011, 0(3) |
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| Authors: | YAO Xu CHEN Zhi-wei ZHANG Cai-yun XIA Xiao-feng YANG Jian-na ZHENG Neng-xiong Fuzhou Center for Disease Control Prevention Fuzhou Fujiang China |
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| Affiliation: | YAO Xu,CHEN Zhi-wei,ZHANG Cai-yun,XIA Xiao-feng,YANG Jian-na,ZHENG Neng-xiong Fuzhou Center for Disease Control and Prevention,Fuzhou,Fujiang 350005,China |
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| Abstract: | Objective To understand the variation characteristics and evolutionary distribution of hemagglutinin gene among H1N1 influenza viruses isolated in 2009.Methods The H1N1 influenza virus was isolated from patients by MDCK cell culture and identified by real-time PCR assay.The hemagglutinin(HA) gene of H1N1 influenza viruses was sequenced for the representative strains in Fuzhou.Finally,the phylogenetic tree was generated and analyzed with DNASTAR MegAlign soft ware.Results The H1N1 influenza virus isolated in... |
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| Keywords: | H1N1 influenza Antigen drift Hemagglutinin Gene sequence |
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