化疗联合G-CSF动员的骨髓及外周血CD34+细胞表达粘附分子的变化

探讨化疗合G－CSF动员前，后患骺髓及外周血CD34^ 细胞表达CD44，CD49d，CD62L及趋化因子CXCR4的动态变化，用免疫荧光直接三角标记和流式细胞术测定G－CSF动员前，后骨髓及外周血CD34^ 细胞的CD44，CD49d，CD62L及趋化因子CXCR4的表达，观察输注各表达亚群细胞数与移植后造血重建的关系。结果发现，动员后骨髓中CD34^ CD44^ 和CD34^＋CD49d^ 细胞的百分比较动员前明显降低, 而外周血中二的比例则显升高；动员前，后骨髓中CD34^＋CD62L^＋和CD34^＋CXCR4^ 细胞的变化并不明显，而外周血中前明显增加，后则显减少。输入CD44^＋，CD49d^ ，CD62L^ 及CXCR4^ 的CD34^＋细胞的量与移植后血中中性粒细胞和血小板恢复的时间未表明有显的相关，结论：G－CSF动员可下调骨髓CD34^＋细胞的CD44，CD49d，CD62L及CXCR4的表达，从而进入外周血循环，输注这些细胞的临床意义有待累积更多病例的分析。

Change of Adhesion Molecule Expression on CD34+ Cells from Bone Marrow and Peripheral Blood during Mobilization with Combination of Chemotherapy and G-CSF

To explore the dynamic change of CD34 + cell expressing adhesion molecules in bone marrow and peripheral blood during mobilization with combination of chemotherapy and G CSF and its clinical significance, mononuclear cells of bone marrow and peripheral blood from malignant hematopathy cases before and after mobilization with G CSF were labeled by CD45 CY Chrome, PE conjugated anti CD34, and FITC conjugated anti CD44, anti CD49d, anti CD62L and anti CXCR4. For three color fluorescence analysis by flow cytometry was performed on a FACScalibur. Also the relationship between the number of subpopulations in different expressions of adhesion molecules infused and the time of recovery in different blood cells after transplantation was evaluated. Results showed that a significantly lower expression of CD44 + and CD49d + on CD34 + cells in bone marrow after mobilization compared to that before mobilization, whereas great higher expression of CD44 +, CD49d +, anti CD62L + and lower of anti CXCR4 + in peripheral blood were observed after mobilization. No significant relations were found between expression of different adhesion molecules on CD34 + cells infused and the time of reconstitution in blood cells after transplantation. It was concluded that this mobilizing regimen could downregulate the expressions of CD44, CD49d, CD62L, and anti CXCR4 on CD34 + cells in bone marrow, it may related to mobilization of CD34 + cells from marrow to blood, and homing of blood CD34 + cells into marrow.