| 高效液相色谱法测定磷酸苯丙哌林胶囊中磷酸苯丙哌林的含量 |
| |
| 引用本文： | 卜生高.高效液相色谱法测定磷酸苯丙哌林胶囊中磷酸苯丙哌林的含量[J].中南药学,2009,7(2):111-113. |
| |
| 作者姓名： | 卜生高 |
| |
| 作者单位： | 湖南省永州市药品检验所,湖南,零陵,425100 |
| |
| 摘 要： | 目的建立高效液相色谱法测定磷酸苯丙哌林胶囊中磷酸苯丙哌林含量的方法。方法用C18色谱柱（4.6mm×250 mm,5μm）,流动相：甲醇-0.1 mol.L-1醋酸铵缓冲液（用冰醋酸调节pH至3.3）（65∶35）,流速：1.0 mL.min-1 检测波长：270 nm 柱温：室温。结果苯丙哌林的线性范围40.5~1012.0μg.mL-1,r=0.9995,平均回收率为99.2%,RSD=0.8%。结论方法简便、可靠、快速。
|
| 关 键 词： | 磷酸苯丙哌林 HPLC 含量测定 |
HPLC determination of benproperine phosphate in benproperine phosphate capsules |
| |
| BU Sheng-gao.HPLC determination of benproperine phosphate in benproperine phosphate capsules[J].Central South Pharmacy,2009,7(2):111-113. |
| |
| Authors: | BU Sheng-gao |
| |
| Institution: | BU Sheng-gao (Institute for Drug Control of Yongzhou , Lingling Hunan , 425100) |
| |
| Abstract: | Objective To establish an HPLC method for the determination of benproperine phosphate in benproperine phosphate capsules. Methods C18 column （4. 6 mm× 250 mm, μm） was used with methanol-0. 1 mol · L-1 ammonium acetic acid buffer solution （adjusted to pH 3. 3 by glacial acetic acid） （65 ： 35） as the mobile phase. The flow rate was 1.0 mL · min-1 and the column temperature was room temperature. The detection wavelength was 270 nm. Results The linear range was 40. 5-1012. 0μg · mL-1 （r=0. 999 5） for benproperine phosphate and average recovery was 99.2%, with RSD=0. 8%. Conclusion The method is simple, reliable, and rapid. |
| |
| Keywords: | HPLC |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
