正常角膜上皮细胞单纯疱疹I型病毒潜伏的研究

目的 检测近视眼患者角膜上皮细胞单纯疱疹Ⅰ型病毒(HSV-Ⅰ)，探讨其在正常角膜潜伏的可能性。方法取行准分子激光屈光性角膜切削术(PRK)的近视眼角膜上皮细胞138份(138眼)，每份均匀分为三部分，两份分别行HSV-Ⅰ的原位聚合酶链反应(ISPCR)检测和间接免疫荧光法(IFA)检测，阳性者取其相对应的第3份角膜上皮进行病毒分离培养和电镜观察。结果 138份近视眼角膜上皮中，ISPCR阳性者8眼(5．8％)，IFA阳性者4眼(2．9％)，两种检测均阳性者3眼(2．2％)，取此9眼的第3份角膜上皮进行病毒分离培养，电镜扫描未发现病毒颗粒。结论正常角膜上皮细胞内存有HSV．I功能基因，提示可能有HSV—I的潜伏。

Experimental study on latency of HSV-I in normal corneal epithelial cell

ObjectiveTo detect the HSV-I in corneal epi t helial cell with myopia and investigate the possibility of the HSV-I hiding in normal cornea.Methods138 corneal samples from 138 myopia eyes were obtained during PRK and each was equally divided into three parts,2 of 3 parts were scree ned by Indirect Immunofluorescence Assay (IFA) and in situ Polymerase Chain Reac tion (ISPCR) to evaluate the possibility of the HSV-I latency.Then the 3rd part of corneas with positive results was used for the isolation of HSV-I and elect ron microscope examination.ResultsEight (5 8%) of the 138 samples were posi tive for HSV-I DNA by ISPCR,4 (2 9%) by IFA,3 (2 2%) by both,and no HSV-I wa s found under electronic microscope.ConclusionHSV-I DNA is positive in norm al corneal epithelial cell,suggesting the possibility of HSV-I latency in norma l cornea.