木犀草素对肝星状细胞迁移和增殖的影响

目的:研究木犀草素对肝星状细胞迁移的影响。方法:体外培养肝星状T6(HSC-T6)细胞,细胞计数Kit8(CCK-8)法检测0、10、20、40μmol/L木犀草素对HSC-T6细胞增殖的影响;通过划痕实验、Transwell实验观察0、10、20、40μmol/L木犀草素对HSC-T6细胞迁移能力的影响;蛋白印迹法检测木犀草素对细胞外信号调节激酶(ERK)5蛋白磷酸化水平的影响。结果:20、40μmol/L木犀草素能明显抑制HSC-T6细胞的增殖(P<0.05);10、20、40μmol/L木犀草素能明显抑制HSC-T6细胞划痕的修复(P<0.05);10、20、40μmol/L木犀草素能明显抑制HSC-T6细胞穿膜(P<0.05);10、20、40μmol/L木犀草素能明显抑制细胞中由血清诱导的去磷酸化(p)-ERK5表达(P<0.05)。结论:木犀草素呈浓度依赖性抑制HSC-T6细胞的增殖和迁移,其机制可能与抑制ERK5蛋白磷酸化水平有关。

Effects of Luteloin on the Migration and Proliferation of Hepatic Stellate Cells

OBJECTIVE： To study the effects of luteolin on the migration of hepatic stellate cells cell line （HSC-T6 cells）. METHODS： HSC-T6 cells were cultured in vitro. CCK-8 assay was used to measure the effects of 0, 10, 20, 40 μmol/L luteolin on the proliferation of HSC-T6 cells; Scratch test and Transwell metastasis experiment were applied to observe the effects of 0, 10, 20, 40 μmol/L luteolin on the migration ability of HSC-T6 cells; phosphorylation level of ERK5 was detected by Western blot. RESULTS： 20, 40 pmol/L luteolin could significantly inhibit the proliferation of HSC-T6 cells（P〈0.05）; 10, 20, 40 μmol/L lute- olin inhibited scratch repair of HSC-T6 cells significantly and decreased the number of HSC-T6 cells passing through cell membrane （P〈0.05） ; the expression of p-ERK5 in HSC-T6 cells was inhibited by 10, 20, 40μmol/L luteolin significantly（P〈0.05）. CONCLUSIONS ： Luteolin inhibit the proliferation and migration of HSC-T6 cells in dose-dependent manner, which may be associated with the inhibition of ERK5 phosphorylation in HSC-T6 cells.