丝瓜叶成分对脑缺血大鼠学习记忆障碍及皮层体感诱发电位的影响

目的:研究丝瓜叶成分L-6a 和L-10 对脑缺血大鼠学习记忆障碍及皮层体感诱发电位(SEP) 的影响。方法:用脑缺血大鼠模型,侧脑室注射(icv)给药,以穿梭箱主动回避反应(AAR) 测定学习记忆行为;用SYD₄₂₂₈ 型生理实验系统测定皮层体感诱发电位。结果:L-6a 0-5 mg·kg^-1 显著促进脑缺血大鼠AAR的获得;0-25 mg·kg^-1 时,无明显影响;L-6a 0-5 及0-25 mg·kg^-1 均显著延缓AAR的消退;脑缺血后,L-6a(0-5 mg·kg^-1) 也减缓SEP波幅下降及其潜伏期延长;脑缺血再通后,L-6a 和L-10(0-5 mg·kg^-1)均加大SEP振幅增高,缩短其潜伏期。结论:L-6a 有显著促进脑障碍大鼠脑功能恢复的作用。

EFFECTS OF CONSTITUENTS L 6a and L 10 FROM LEAVES OF LUFFA CYLINDRICA ROEM ON DISFUNCTION OF LEARNING MEMORY AND SEP OF CEREBRAL ISCHMEIA RATS

To study the effects of the constituents L 6a and L 10 from leaves of Luffa cylindrica Roem on learning memory disfunction and seduce electric potential (SEP) in cerebral ischemia rats. METHODS: The learning memory behavior was determined by active avoidance response (AAR) in shuttle box and the seduce electric potential (SEP) in cortical body sensation region was measured by SYD₄₂₂₈ physiological experiment system in model of cerebral ischemia rat after injecting experimental drugs in cerebral ventricle (icv). RESULTS: Treatment with L-6a at dosage of 0.5 mg·kg^-1 obviously promoted acquirement of AAR in cerebral ischemia rats (P<0.05) and at doses of 0.5 and 0.25 mg·kg^-1 obviously postponed the fading away of AAR (P<0.05). Treatment with L-6a (0.5 mg·kg^-1 ) was shown to relief the descent of SEP wave amplitude and the prolongation of SEP latent period after cerebral ischemia (P<0.05). When blood was poured into brain again, both L-6a and L-10 were shown to markedly amplify SEP wave amplitude (P<0.05) and shorten latent period of SEP. CONCLUSION: L-6a was found to obviously promote recovery of cerebral disfunction in rats.