虎杖、何首乌和掌叶大黄、唐古特大黄的1HNMR指纹图解析

目的:对中药材虎杖、何首乌和掌叶大黄、唐古特大黄的¹HNMR 指纹图进行解析研究,并比较蓼属与大黄属植物¹HNMR 指纹图的差别。方法:应用硅胶柱色谱法和结晶法分离特征化学成分,对单体化合物进行¹HNMR研究,从而实现中药材¹HNMR 指纹图的解析。结果:从虎杖和何首乌特征总提物A 中均分离出大黄素甲醚,其¹HNMR 指纹图均主要显示大黄素甲醚的特征共振峰,两者既有相似性,又有一定的差别。从两种大黄属植物特征总提物A中均分离出大黄酚和大黄素甲醚,其特征总提物A 的¹HNMR指纹图均主要显示大黄酚和大黄素甲醚的特征共振峰。蓼属与大黄属植物的¹HNMR 指纹图既存在相同的部分,又有本质区别。结论:¹HNMR 指纹图可用于虎杖和何首乌、掌叶大黄和唐古特大黄的品种鉴别及蓼属和大黄属植物的分类鉴别。

ASSIGNMENTS OF THE 1HNMR FINGER PRINTS OF THE ROOTS OF POLYGONUM CUSPIDATUM , POLYGONUM MULTIFLORUM , RHEUM PALMATUM AND RHEUM TANGUTICUM

AIM: To assign the ¹HNMR finger prints of the roots of Polygonum cuspidatum, Polygonum multiflorum, Rheum palmatum and Rheum tanguticum, and to compare the differences of the ¹HNMR finger prints between Polygonum and Rheum species. METHODS: After the characteristic chemical constituents of the extracts of the above traditional Chinese herbal medicines isolated by silica gel column chromatography and crystallization, their ¹HNMR spectra were investigated, and the characteristic signals of every ¹HNMR finger prints were assigned. RESULTS: Physcion was isolated from the characteristic general extracts A of the roots of P. cuspidatum and P. multiflorum , physcion-8-O-β-D glucoside from extracts B of the above two species, and chryxophanol and physcion from extract A of the roots of R. palmatum and R.tanguticum. The ¹HNMR finger prints of the roots of P.cuspidatum and P. multiflorum which belong to the same genus had high similarity and showed mainly the characteristic signals of physcion. On the other hand, owing to the ratio discrepancy of the major similar constituents, the relative intensity of ¹HNMR signals showed obvious differences. The ¹HNMR finger print B of P. cuspidatum showed mainly the characteristic signals of physcion-8-O-β-D glucoside, but similar signals were not observed from the same extract of P. multiflorum. Both the same aspects and the essential differences could be observed from the ¹HNMR finger prints of the Rheum and Polygonum species. CONCLUSION: The ¹HNMR finger prints might be utilized for the identification of the roots of P. cuspidatum and P. multiflorum, Rheum palmatum and Rheum tanguticum, and the classification of Polygonum and Rheum species.