17β-雌二醇对人的类成骨细胞株TE85和U2功能的调节

目的：以人的类成骨细胞株TE85和U2为细胞模型，观察雌激素对细胞功能的影响。方法：³H-胸腺嘧啶参入法测细胞增殖；ELISA法测细胞因子IL-6含量；精氨酸转化法测iNOS活性。结果：IL-1(20 U.mL^-1)和TNFα(50 U.mL^-1)刺激细胞分泌IL-6，并抑制细胞增殖。在TE85细胞，17β-雌二醇(E₂)可抑制IL-6分泌并能对抗IL-1和TNFα对细胞增殖的影响。NOS抑制剂L-NMMA使细胞³H-胸腺嘧啶的参入降低，E₂(1 nmol.L^-1)可对抗L-NMMA的抑制作用。结论：E₂通过减少IL-6分泌和影响iNOS活性而调节细胞功能。

REGULATION OF 17β-ESTRADIOL IN HUMAN OSTEOBLAST-LIKE CELL LINES TE85 AND U2

AIM: Using two kinds of osteoblast-like cell lines TE85 and U2 as cell models, the molecular mechanism of 17β-estradiol (E₂) in regulating cell function was studied. METHODS: Using methods of ³H-thymidine incorporation for cell proliferation, ELISA for IL-6 content measurement and transferring L-³H-arginine to L-³H-citruline for iNOS activity. RESULTS: In two cell lines，interleukin-1(IL-1, 20 U*mL-1) and tumor necrosis factor (TNFα 50 U.mL^-1) stimulated osteoblast to secret IL-6 after treatment for 48 h. At the same concentration, IL-1 and TNFα reduced the intracellular protein content resulting from cell proliferation inhibition. In TE85 cells, E₂ reduced the IL-6 secretion and inhibited the influence of IL-1 and TNFα on the cell proliferation. After L-NMMA, an inhibitor of NOS, was added, the ³H-TdR uptake decreased in a dose-dependent fashion in TE85 and U2 cells. When the cells were treated with E₂ and L-NMMA concomitantly, the inhibiting effects of the latter were diminished and the percentage of ³H-TdR uptake reached normal levels. Besides that, IL-1 and TNFα increased intracellular iNOS activity significantly, which was higher than that of E₂. CONCLUSION: E₂ regulated cell function by way of reducing IL-6 secretion and improving iNOS activity.