依布硒啉对四氯化碳及内毒素+D-氨基半乳糖致肝损伤的保护作用

目的：考察依布硒啉(ebselen)对肝损伤的保护作用。方法：以0.2%四氯化碳(5 ml.kg^-1)和内毒素（1μg.kg^-1)+D-氨基半乳糖（800 mg.kg^-1)分别ip ICR小鼠形成肝损伤，观察病理改变及血清谷丙转氨酶(ALT)、总胆红素(TBIL)和总胆汁酸(TBA)活性。培养大鼠肝细胞以CCl₄和LPS＋D-GalN诱发损伤。测定了乳酸脱氢酶活性和细胞TBARS含量。结果：依伯硒啉可改善CCl₄和LPS＋D-GalN诱发的小鼠肝损伤变化，减小相关损伤指标的改变；并可拮抗培养肝细胞的LDH释放和TBARS含量上升。结论：依布硒啉能保护肝损伤，可能与抗自由基的脂质过氧化有关。

PROTECTION OF EBSELEN ON LIVER DAMAGE INDUCED BY CCL4 AND LIPOPOLYSACCHARIDES+D-GALACTOSAMINE IN VIVO AND IN VITRO

AIM: To study the protective effect of ebselen on liver damage. METHODS: ICR mice and cultured hepatocytes were both treated with CCl 4 and lipopolysaccharide D galactosamine. Pathological changes and glutamine pyruvic transaminase(ALT), total bile acid(TBA)as well as total bilirubin(TBIL)contents in mouse serum were determined. RESULTS: The LDH activity in supernatant and thiobarbituric acid reactive substance(TBARS) content of cultured hepatocytes were evaluated. Results showed that ebselen ameliorated the pathological changes of the mouse liver and decreased the contents of ALT, TBA and TBIL. The LDH efflux and TBARS increase in hepatocytes were also depressed by ebselen. CONCLUSION: The above results indicate that ebselen is effective against liver damage, which may derive from its anti lipid oxidation capacity.